Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
Title:
Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
Author:
Butterick, Tammy A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (507 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51305