In vivo Laser Axotomy in C. elegans
Title:
In vivo Laser Axotomy in C. elegans
Author:
Byrne, Alexandra B.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (558 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2707