Search Results for - Narrowed by: 2016 - Genetics

A Virtual Machine Platform for Non-Computer Professionals for Using Deep Learning to Classify Biological Sequences of Metagenomic Data

2024-05-31T01:26:23Z

by Fang, Zhencheng
https://www.jove.com/t/62250
Format: Visual Materials

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

2024-05-31T01:26:23Z

by Liu, Siming
https://www.jove.com/t/59578
Format: Visual Materials

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

2024-05-31T01:26:23Z

by Shaw, Jeff A.
https://www.jove.com/t/59630
Format: Visual Materials

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

2024-05-31T01:26:23Z

by Šafarič Tepeš, Polona
https://www.jove.com/t/60685
Format: Visual Materials

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

2024-05-31T01:26:23Z

by Wu, Zechi
https://www.jove.com/t/62423
Format: Visual Materials

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

2024-05-31T01:26:23Z

by Chiola, Simone
https://www.jove.com/t/59242
Format: Visual Materials

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

2024-05-31T01:26:23Z

by Yamakawa, Takahiro
https://www.jove.com/t/59375
Format: Visual Materials

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

2024-05-31T01:26:23Z

by Korosec, Ana
https://www.jove.com/t/59372
Format: Visual Materials

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

2024-05-31T01:26:23Z

by Almeida, Camila F.
https://www.jove.com/t/61991
Format: Visual Materials

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

2024-05-31T01:26:23Z

by Wang, Yuyang
https://www.jove.com/t/59428
Format: Visual Materials

Screening Cotton Genotypes for Reniform Nematode Resistance

2024-05-31T01:26:23Z

by Erpelding, John E.
https://www.jove.com/t/58577
Format: Visual Materials

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

2024-05-31T01:26:23Z

by Dix, Christie
https://www.jove.com/t/61506
Format: Visual Materials

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

2024-05-31T01:26:23Z

by Yanai, Katsunori
https://www.jove.com/t/61383
Format: Visual Materials

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

2024-05-31T01:26:23Z

by Pajič, Tadej
https://www.jove.com/t/61642
Format: Visual Materials

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

2024-05-31T01:26:23Z

by Loganathan, Sampath Kumar
https://www.jove.com/t/61693
Format: Visual Materials

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

2024-05-31T01:26:23Z

by Haftbaradaran Esfahani, Payam
https://www.jove.com/t/61577
Format: Visual Materials

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

2024-05-31T01:26:23Z

by Levin, Yelena
https://www.jove.com/t/61060
Format: Visual Materials

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

2024-05-31T01:26:23Z

by Sharma, Sumana
https://www.jove.com/t/60803
Format: Visual Materials

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

2024-05-31T01:26:23Z

by Thrash, Adam
https://www.jove.com/t/61268
Format: Visual Materials

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

2024-05-31T01:26:23Z

by Shi, Jinxia
https://www.jove.com/t/60697
Format: Visual Materials

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

2024-05-31T01:26:23Z

by Sinha, Snehadri
https://www.jove.com/t/60705
Format: Visual Materials

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

2024-05-31T01:26:23Z

by Noe Gonzalez, Melvin
https://www.jove.com/t/59497
Format: Visual Materials

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

2024-05-31T01:26:23Z

by Poulton, Beth Crawford
https://www.jove.com/t/62131
Format: Visual Materials

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

2024-05-31T01:26:23Z

by Sharon, Belle M.
https://www.jove.com/t/62872
Format: Visual Materials

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

2024-05-31T01:26:23Z

by Fernandes, L. G. V.
https://www.jove.com/t/62631
Format: Visual Materials

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

2024-05-31T01:26:23Z

by Smith, Amy
https://www.jove.com/t/62001
Format: Visual Materials

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

2024-05-31T01:26:23Z

by Timme, Kelsey R.
https://www.jove.com/t/59414
Format: Visual Materials

Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

2024-05-31T01:26:23Z

by Louradour, Isabelle
https://www.jove.com/t/61924
Format: Visual Materials

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

2024-05-31T01:26:23Z

by Shaver, Amanda O.
https://www.jove.com/t/61453
Format: Visual Materials

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

2024-05-31T01:26:23Z

by Smits, Jos G.A.
https://www.jove.com/t/60905
Format: Visual Materials

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

2024-05-31T01:26:23Z

by Saplaoura, Eleftheria
https://www.jove.com/t/61231
Format: Visual Materials

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

2024-05-31T01:26:23Z

by Chauve, Laetitia
https://www.jove.com/t/61132
Format: Visual Materials

High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)

2024-05-31T01:26:23Z

by Montanera, Kaitlin N.
https://www.jove.com/t/61124
Format: Visual Materials

In-Nucleus Hi-C in Drosophila Cells

2024-05-31T01:26:23Z

by Esquivel-López, Ayerim
https://www.jove.com/t/62106
Format: Visual Materials

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

2024-05-31T01:26:23Z

by Carballar-Lejarazú, Rebeca
https://www.jove.com/t/62607
Format: Visual Materials

Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes

2024-05-31T01:26:23Z

by Carballar-Lejarazú, Rebeca
https://www.jove.com/t/62588
Format: Visual Materials

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

2024-05-31T01:26:23Z

by Guo, Jing-Ying
https://www.jove.com/t/57351
Format: Visual Materials

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

2024-05-31T01:26:23Z

by Farboud, Behnom
https://www.jove.com/t/57350
Format: Visual Materials

Improving Animal Welfare through Genetic Selection

2024-05-31T01:26:23Z

by Wendy Mercedes Rauw
DOAB: download the publication DOAB: description of the publication
Format: Books

Why vaccines to HIV, HCV and Malaria have so far failed - Challenges to developing vaccines against immunoregulating pathogens

2024-05-31T01:26:23Z

by Magdalena Plebanski
DOAB: download the publication DOAB: description of the publication
Format: Books

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

2024-05-31T01:26:23Z

by Siebers, Meike
https://www.jove.com/t/62305
Format: Visual Materials

Microinjection Method for Anopheles gambiae Embryos

2024-05-31T01:26:23Z

by Carballar-Lejarazú, Rebeca
https://www.jove.com/t/62591
Format: Visual Materials

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

2024-05-31T01:26:23Z

by Taye, Nandaraj
https://www.jove.com/t/60824
Format: Visual Materials

Identification of Circular RNAs using RNA Sequencing

2024-05-31T01:26:23Z

by Sekar, Shobana
https://www.jove.com/t/59981
Format: Visual Materials

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

2024-05-31T01:26:23Z

by Ma, Mengxiao
https://www.jove.com/t/60413
Format: Visual Materials

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

2024-05-31T01:26:23Z

by Harnish, J. Michael
https://www.jove.com/t/59658
Format: Visual Materials

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

2024-05-31T01:26:23Z

by Wang, Julia
https://www.jove.com/t/59542
Format: Visual Materials

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

2024-05-31T01:26:23Z

by Immarigeon, Clément
https://www.jove.com/t/60218
Format: Visual Materials

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

2024-05-31T01:26:23Z

by Wilson, Andi M.
https://www.jove.com/t/61367
Format: Visual Materials

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

2024-05-31T01:26:23Z

by Takahashi, Hazuki
https://www.jove.com/t/58627
Format: Visual Materials

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

2024-05-31T01:26:23Z

by Wang, Jingxin
https://www.jove.com/t/59021
Format: Visual Materials

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

2024-05-31T01:26:23Z

by Pollpeter, Darja
https://www.jove.com/t/58715
Format: Visual Materials

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

2024-05-31T01:26:23Z

by Behera, Ranjan K.
https://www.jove.com/t/59055
Format: Visual Materials

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

2024-05-31T01:26:23Z

by Radtke, Stefan
https://www.jove.com/t/58933
Format: Visual Materials

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

2024-05-31T01:26:23Z

by Shrestha, Shaleen
https://www.jove.com/t/59192
Format: Visual Materials

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

2024-05-31T01:26:23Z

by Paschke, Peggy
https://www.jove.com/t/58981
Format: Visual Materials

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

2024-05-31T01:26:23Z

by Miller, Jessica M.
https://www.jove.com/t/58971
Format: Visual Materials

Novel Sequence Discovery by Subtractive Genomics

2024-05-31T01:26:23Z

by Asalone, Kathryn C.
https://www.jove.com/t/58877
Format: Visual Materials

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

2024-05-31T01:26:23Z

by Bondy, Elizabeth C.
https://www.jove.com/t/59213
Format: Visual Materials

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

2024-05-31T01:26:23Z

by Fiedler, Isabell-Christin
https://www.jove.com/t/58746
Format: Visual Materials

Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry

2024-05-31T01:26:23Z

by Riddle, Misty
https://www.jove.com/t/58972
Format: Visual Materials

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

2024-05-31T01:26:23Z

by Wadosky, Kristine M.
https://www.jove.com/t/59710
Format: Visual Materials

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

2024-05-31T01:26:23Z

by De Iuliis, Vincenzo
https://www.jove.com/t/57289
Format: Visual Materials

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

2024-05-31T01:26:23Z

by Luo, Huacheng
https://www.jove.com/t/59382
Format: Visual Materials

A Customizable Protocol for String Assembly gRNA Cloning (STAgR)

2024-05-31T01:26:23Z

by Breunig, Christopher T.
https://www.jove.com/t/58556
Format: Visual Materials

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

2024-05-31T01:26:23Z

by Avogaro, Laura
https://www.jove.com/t/58790
Format: Visual Materials

Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation

2024-05-31T01:26:23Z

by Jones, Edward G
https://www.jove.com/t/58907
Format: Visual Materials

Purification of Low-abundant Cells in the Drosophila Visual System

2024-05-31T01:26:23Z

by Peng, Jing
https://www.jove.com/t/58474
Format: Visual Materials

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

2024-05-31T01:26:23Z

by Tagliafierro, Lidia
https://www.jove.com/t/59241
Format: Visual Materials

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

2024-05-31T01:26:23Z

by Vander Schaaf, Nicole A.
https://www.jove.com/t/59235
Format: Visual Materials

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

2024-05-31T01:26:23Z

by Decraene, Charles
https://www.jove.com/t/58051
Format: Visual Materials

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

2024-05-31T01:26:23Z

by Keit, Emily
https://www.jove.com/t/58918
Format: Visual Materials

Culturing and Manipulation of O9-1 Neural Crest Cells

2024-05-31T01:26:23Z

by Nguyen, Bao H.
https://www.jove.com/t/58346
Format: Visual Materials

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

2024-05-31T01:26:23Z

by Dussaud, Sébastien
https://www.jove.com/t/57609
Format: Visual Materials

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

2024-05-31T01:26:23Z

by Sommarin, Mikael N.E.
https://www.jove.com/t/57831
Format: Visual Materials

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

2024-05-31T01:26:23Z

by Brettmann, Erin A.
https://www.jove.com/t/58030
Format: Visual Materials

CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum

2024-05-31T01:26:23Z

by Kudyba, Heather M.
https://www.jove.com/t/57747
Format: Visual Materials

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

2024-05-31T01:26:23Z

by Wang, Guannan
https://www.jove.com/t/58544
Format: Visual Materials

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

2024-05-31T01:26:23Z

by Li, Ming
https://www.jove.com/t/56990
Format: Visual Materials

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

2024-05-31T01:26:23Z

by Davari, Kathrin
https://www.jove.com/t/56752
Format: Visual Materials

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

2024-05-31T01:26:23Z

by Chesner, Lisa N.
https://www.jove.com/t/57413
Format: Visual Materials

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

2024-05-31T01:26:23Z

by Porter, Elizabeth G.
https://www.jove.com/t/55369
Format: Visual Materials

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

2024-05-31T01:26:23Z

by Fang, Mingzhu
https://www.jove.com/t/55832
Format: Visual Materials

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

2024-05-31T01:26:23Z

by Isomura, Akihiro
https://www.jove.com/t/57149
Format: Visual Materials

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

2024-05-31T01:26:23Z

by Toh, Desiree-Faye Kaixin
https://www.jove.com/t/56221
Format: Visual Materials

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation

2024-05-31T01:26:23Z

by Shirley, Ben
https://www.jove.com/t/56245
Format: Visual Materials

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

2024-05-31T01:26:23Z

by Blitz, Ira L.
https://www.jove.com/t/56035
Format: Visual Materials

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

2024-05-31T01:26:23Z

by Jandura, Allison
https://www.jove.com/t/56281
Format: Visual Materials

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

2024-05-31T01:26:23Z

by Zhang, Pingyu
https://www.jove.com/t/55689
Format: Visual Materials

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

2024-05-31T01:26:23Z

by Romano, Oriana
https://www.jove.com/t/55919
Format: Visual Materials

Generation of Fluorescent Protein Fusions in Candida Species

2024-05-31T01:26:23Z

by Gonia, Sara
https://www.jove.com/t/55333
Format: Visual Materials

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

2024-05-31T01:26:23Z

by Reichel, Jonathan B.
https://www.jove.com/t/54399
Format: Visual Materials

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

2024-05-31T01:26:23Z

by Timpano, Sara
https://www.jove.com/t/55112
Format: Visual Materials

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

2024-05-31T01:26:23Z

by Xiang, Jie
https://www.jove.com/t/54976
Format: Visual Materials

In Vitro Transcription Assays and Their Application in Drug Discovery

2024-05-31T01:26:23Z

by Yang, Xiao
https://www.jove.com/t/54256
Format: Visual Materials

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

2024-05-31T01:26:23Z

by Lokossou, Adjimon Gatien
https://www.jove.com/t/53995
Format: Visual Materials

Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

2024-05-31T01:26:23Z

by Faris, Robert
https://www.jove.com/t/60488
Format: Visual Materials

Mapping Alzheimer's Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

2024-05-31T01:26:23Z

by Matoba, Nana
https://www.jove.com/t/60428
Format: Visual Materials

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

2024-05-31T01:26:23Z

by Wardhani, Titis A.K.
https://www.jove.com/t/59971
Format: Visual Materials

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

2024-05-31T01:26:23Z

by Xin, Yurong
https://www.jove.com/t/59866
Format: Visual Materials

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

2024-05-31T01:26:23Z

by Bennett, Seth A.
https://www.jove.com/t/59104
Format: Visual Materials

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

2024-05-31T01:26:23Z

by Pourshafie, Naemeh
https://www.jove.com/t/55724
Format: Visual Materials

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

2024-05-31T01:26:23Z

by Zhou, Dan
https://www.jove.com/t/58467
Format: Visual Materials

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

2024-05-31T01:26:23Z

by Jayaraman, Jyothi
https://www.jove.com/t/58646
Format: Visual Materials

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

2024-05-31T01:26:23Z

by Kao, Shao-Yen
https://www.jove.com/t/60309
Format: Visual Materials

Simultaneous Video-EEG-ECG Monitoring to Identify Neurocardiac Dysfunction in Mouse Models of Epilepsy

2024-05-31T01:26:23Z

by Mishra, Vikas
https://www.jove.com/t/57300
Format: Visual Materials

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

2024-05-31T01:26:23Z

by Hashemipetroudi, Seyyed Hamidreza
https://www.jove.com/t/55451
Format: Visual Materials

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

2024-05-31T01:26:23Z

by Elaswad, Ahmed
https://www.jove.com/t/56275
Format: Visual Materials

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

2024-05-31T01:26:23Z

by Rehimi, Rizwan
https://www.jove.com/t/56186
Format: Visual Materials

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

2024-05-31T01:26:23Z

by Rivera-Torres, Natalia
https://www.jove.com/t/56195
Format: Visual Materials

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

2024-05-31T01:26:23Z

by Chen, Yuhui
https://www.jove.com/t/56470
Format: Visual Materials

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

2024-05-31T01:26:23Z

by Hubner, Eric K.
https://www.jove.com/t/56613
Format: Visual Materials

The Nematode Caenorhabditis Elegans - A Versatile In Vivo Model to Study Host-microbe Interactions

2024-05-31T01:26:23Z

by Issi, Luca
https://www.jove.com/t/56487
Format: Visual Materials

Quantifying Abdominal Pigmentation in Drosophila melanogaster

2024-05-31T01:26:23Z

by Saleh Ziabari, Omid
https://www.jove.com/t/55732
Format: Visual Materials

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

2024-05-31T01:26:23Z

by Shen, Bang
https://www.jove.com/t/55185
Format: Visual Materials

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

2024-05-31T01:26:23Z

by Gross, Jonas
https://www.jove.com/t/55501
Format: Visual Materials

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

2024-05-31T01:26:23Z

by Giaimo, Benedetto Daniele
https://www.jove.com/t/55907
Format: Visual Materials

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

2024-05-31T01:26:23Z

by Sternburg, Erin L.
https://www.jove.com/t/55903
Format: Visual Materials

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

2024-05-31T01:26:23Z

by Ramsawhook, Ashley H.
https://www.jove.com/t/56318
Format: Visual Materials

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

2024-05-31T01:26:23Z

by Erdogan, Fettah
https://www.jove.com/t/54854
Format: Visual Materials

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

2024-05-31T01:26:23Z

by Joyce, Blake L.
https://www.jove.com/t/55009
Format: Visual Materials

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

2024-05-31T01:26:23Z

by Apraiz, Aintzane
https://www.jove.com/t/55745
Format: Visual Materials

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

2024-05-31T01:26:23Z

by Breker, Michal
https://www.jove.com/t/54831
Format: Visual Materials

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

2024-05-31T01:26:23Z

by Gall, Joseph G.
https://www.jove.com/t/54103
Format: Visual Materials

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

2024-05-31T01:26:23Z

by Greule, Anja
https://www.jove.com/t/54952
Format: Visual Materials

Metagenomic Analysis of Silage

2024-05-31T01:26:23Z

by Tennant, Richard K.
https://www.jove.com/t/54936
Format: Visual Materials

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

2024-05-31T01:26:23Z

by Turton, Keren B.
https://www.jove.com/t/54473
Format: Visual Materials

Methyl-binding DNA capture Sequencing for Patient Tissues

2024-05-31T01:26:23Z

by Jadhav, Rohit R.
https://www.jove.com/t/54131
Format: Visual Materials

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

2024-05-31T01:26:23Z

by Dal Santo, Silvia
https://www.jove.com/t/54410
Format: Visual Materials

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

2024-05-31T01:26:23Z

by Dhoondia, Zuzer
https://www.jove.com/t/55446
Format: Visual Materials

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

2024-05-31T01:26:23Z

by Gaebler, Anne-Marlen
https://www.jove.com/t/60704
Format: Visual Materials

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

2024-05-31T01:26:23Z

by Marasca, Federica
https://www.jove.com/t/60712
Format: Visual Materials

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

2024-05-31T01:26:23Z

by van Dijk, Erwin L.
https://www.jove.com/t/60056
Format: Visual Materials

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

2024-05-31T01:26:23Z

by Chan, Katherine
https://www.jove.com/t/59780
Format: Visual Materials

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

2024-05-31T01:26:23Z

by Dash, Sabyasachi
https://www.jove.com/t/57786
Format: Visual Materials

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

2024-05-31T01:26:23Z

by Maida, Yoshiko
https://www.jove.com/t/57021
Format: Visual Materials

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

2024-05-31T01:26:23Z

by Gaudreau-Lapierre, Antoine
https://www.jove.com/t/57410
Format: Visual Materials

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

2024-05-31T01:26:23Z

by Huang, Ling
https://www.jove.com/t/57192
Format: Visual Materials

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

2024-05-31T01:26:23Z

by Masamha, Chioniso Patience
https://www.jove.com/t/57318
Format: Visual Materials

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

2024-05-31T01:26:23Z

by Tark-Dame, Mariliis
https://www.jove.com/t/57295
Format: Visual Materials

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

2024-05-31T01:26:23Z

by Chang, Ju-Chun
https://www.jove.com/t/56693
Format: Visual Materials

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

2024-05-31T01:26:23Z

by Yao, Xuan
https://www.jove.com/t/56844
Format: Visual Materials

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

2024-05-31T01:26:23Z

by Leko, Vid
https://www.jove.com/t/56398
Format: Visual Materials

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

2024-05-31T01:26:23Z

by Emetu, Sophia
https://www.jove.com/t/56898
Format: Visual Materials

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

2024-05-31T01:26:23Z

by Murata, Takatoshi
https://www.jove.com/t/56319
Format: Visual Materials

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

2024-05-31T01:26:23Z

by Grünberg, Sebastian
https://www.jove.com/t/55836
Format: Visual Materials

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

2024-05-31T01:26:23Z

by Kisserli, Aymric
https://www.jove.com/t/56012
Format: Visual Materials

Single Molecule Analysis of Laser Localized Psoralen Adducts

2024-05-31T01:26:23Z

by Huang, Jing
https://www.jove.com/t/55541
Format: Visual Materials

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

2024-05-31T01:26:23Z

by Yehuda, Yishai
https://www.jove.com/t/55157
Format: Visual Materials

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

2024-05-31T01:26:23Z

by Perchet, Thibaut
https://www.jove.com/t/54858
Format: Visual Materials

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

2024-05-31T01:26:23Z

by Willis, Stephen D.
https://www.jove.com/t/56226
Format: Visual Materials

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

2024-05-31T01:26:23Z

by Chen, Zhe
https://www.jove.com/t/54516
Format: Visual Materials

Controllable Ion Channel Expression through Inducible Transient Transfection

2024-05-31T01:26:23Z

by Geron, Matan
https://www.jove.com/t/55370
Format: Visual Materials

Detection of Copy Number Alterations Using Single Cell Sequencing

2024-05-31T01:26:23Z

by Knouse, Kristin A.
https://www.jove.com/t/55143
Format: Visual Materials

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

2024-05-31T01:26:23Z

by Farwell, Sara Lynn N.
https://www.jove.com/t/55053
Format: Visual Materials

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

2024-05-31T01:26:23Z

by Lorzadeh, Alireza
https://www.jove.com/t/56085
Format: Visual Materials

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

2024-05-31T01:26:23Z

by Chuah, Jo-Ann
https://www.jove.com/t/54972
Format: Visual Materials

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

2024-05-31T01:26:23Z

by Siano, Giacomo
https://www.jove.com/t/59988
Format: Visual Materials

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

2024-05-31T01:26:23Z

by Wang, Ying
https://www.jove.com/t/59864
Format: Visual Materials

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

2024-05-31T01:26:23Z

by Wein, Tanita
https://www.jove.com/t/60749
Format: Visual Materials

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

2024-05-31T01:26:23Z

by Gui, Baoheng
https://www.jove.com/t/59273
Format: Visual Materials

Use of Drosophila S2 Cells for Live Imaging of Cell Division

2024-05-31T01:26:23Z

by Dewey, Evan B.
https://www.jove.com/t/60049
Format: Visual Materials

A Method to Study de novo Formation of Chromatin Domains

2024-05-31T01:26:23Z

by Oksuz, Ozgur
https://www.jove.com/t/60039
Format: Visual Materials

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

2024-05-31T01:26:23Z

by Course, Meredith M.
https://www.jove.com/t/59901
Format: Visual Materials

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

2024-05-31T01:26:23Z

by Benitez, Gabrielle J.
https://www.jove.com/t/61230
Format: Visual Materials

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

2024-05-31T01:26:23Z

by Cvetesic, Nevena
https://www.jove.com/t/59805
Format: Visual Materials

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

2024-05-31T01:26:23Z

by Li, Shan
https://www.jove.com/t/59960
Format: Visual Materials

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

2024-05-31T01:26:23Z

by Cheng, Chi-Keung
https://www.jove.com/t/60130
Format: Visual Materials

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

2024-05-31T01:26:23Z

by Ashley, Sarah E.
https://www.jove.com/t/57601
Format: Visual Materials

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

2024-05-31T01:26:23Z

by Hug, Clemens B.
https://www.jove.com/t/57001
Format: Visual Materials

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

2024-05-31T01:26:23Z

by Bartolone, Sarah N.
https://www.jove.com/t/58436
Format: Visual Materials

Mating-based Overexpression Library Screening in Yeast

2024-05-31T01:26:23Z

by Hayden, Elliott
https://www.jove.com/t/57978
Format: Visual Materials

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

2024-05-31T01:26:23Z

by Cardenas-Diaz, Fabian L.
https://www.jove.com/t/60085
Format: Visual Materials

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

2024-05-31T01:26:23Z

by Singh, Ravinder
https://www.jove.com/t/57816
Format: Visual Materials

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

2024-05-31T01:26:23Z

by Fritsch, Clark
https://www.jove.com/t/57731
Format: Visual Materials

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

2024-05-31T01:26:23Z

by Tarang, Shikha
https://www.jove.com/t/58419
Format: Visual Materials

The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster

2024-05-31T01:26:23Z

by Lowman, Kelsey E.
https://www.jove.com/t/57788
Format: Visual Materials

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

2024-05-31T01:26:23Z

by Schlaffner, Christoph N.
https://www.jove.com/t/57633
Format: Visual Materials

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

2024-05-31T01:26:23Z

by Yuan, Zhihong
https://www.jove.com/t/57737
Format: Visual Materials

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

2024-05-31T01:26:23Z

by Wambaugh, Morgan A.
https://www.jove.com/t/57241
Format: Visual Materials

Methodology for Accurate Detection of Mitochondrial DNA Methylation

2024-05-31T01:26:23Z

by Mechta, Mie
https://www.jove.com/t/57772
Format: Visual Materials

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

2024-05-31T01:26:23Z

by Seo, Hogyu David
https://www.jove.com/t/57499
Format: Visual Materials

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

2024-05-31T01:26:23Z

by Lee, Seoghyun
https://www.jove.com/t/57494
Format: Visual Materials

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

2024-05-31T01:26:23Z

by Loudig, Olivier
https://www.jove.com/t/57471
Format: Visual Materials

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

2024-05-31T01:26:23Z

by Simmons, Tuesday
https://www.jove.com/t/57561
Format: Visual Materials

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

2024-05-31T01:26:23Z

by Escudero, Jose Antonio
https://www.jove.com/t/57386
Format: Visual Materials

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

2024-05-31T01:26:23Z

by Brunetti, Lorenzo
https://www.jove.com/t/57278
Format: Visual Materials

Production and Purification of Baculovirus for Gene Therapy Application

2024-05-31T01:26:23Z

by Nasimuzzaman, Md
https://www.jove.com/t/57019
Format: Visual Materials

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

2024-05-31T01:26:23Z

by Dilliott, Allison A.
https://www.jove.com/t/57266
Format: Visual Materials

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

2024-05-31T01:26:23Z

by Rodríguez-Gil, Alfonso
https://www.jove.com/t/57272
Format: Visual Materials

Manipulation of Ploidy in Caenorhabditis elegans

2024-05-31T01:26:23Z

by Clarke, Erlyana K.
https://www.jove.com/t/57296
Format: Visual Materials

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

2024-05-31T01:26:23Z

by Uwase, Grace
https://www.jove.com/t/56728
Format: Visual Materials

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

2024-05-31T01:26:23Z

by Köferle, Anna
https://www.jove.com/t/56264
Format: Visual Materials

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

2024-05-31T01:26:23Z

by Jezek, Meagan
https://www.jove.com/t/57080
Format: Visual Materials

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

2024-05-31T01:26:23Z

by Saeidi, Saman
https://www.jove.com/t/56837
Format: Visual Materials

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

2024-05-31T01:26:23Z

by Hostelley, Timothy L.
https://www.jove.com/t/56187
Format: Visual Materials

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

2024-05-31T01:26:23Z

by Grbesa, Ivana
https://www.jove.com/t/56313
Format: Visual Materials

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

2024-05-31T01:26:23Z

by Kong, Xiangduo
https://www.jove.com/t/56213
Format: Visual Materials

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

2024-05-31T01:26:23Z

by Hirai, Keiji
https://www.jove.com/t/55505
Format: Visual Materials

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

2024-05-31T01:26:23Z

by Hassan, Mohamed I.
https://www.jove.com/t/55187
Format: Visual Materials

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

2024-05-31T01:26:23Z

by Shi, Zhong-Dong
https://www.jove.com/t/55267
Format: Visual Materials

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

2024-05-31T01:26:23Z

by Feehan, Joanna M.
https://www.jove.com/t/55463
Format: Visual Materials

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

2024-05-31T01:26:23Z

by Aw, Jong Ghut Ashley
https://www.jove.com/t/55255
Format: Visual Materials

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

2024-05-31T01:26:23Z

by Patel, Dhaval S
https://www.jove.com/t/56292
Format: Visual Materials

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

2024-05-31T01:26:23Z

by Wei, Huan-Huan
https://www.jove.com/t/54967
Format: Visual Materials

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

2024-05-31T01:26:23Z

by Suryawanshi, Gajendra W.
https://www.jove.com/t/55812
Format: Visual Materials

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

2024-05-31T01:26:23Z

by Duina, Andrea A.
https://www.jove.com/t/55263
Format: Visual Materials

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

2024-05-31T01:26:23Z

by Zhang, Lin
https://www.jove.com/t/60917
Format: Visual Materials

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

2024-05-31T01:26:23Z

by Oude Munnink, Bas B.
https://www.jove.com/t/60906
Format: Visual Materials

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

2024-05-31T01:26:23Z

by Weiss, Carly V.
https://www.jove.com/t/59972
Format: Visual Materials

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

2024-05-31T01:26:23Z

by Lietard, Jory
https://www.jove.com/t/59936
Format: Visual Materials

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

2024-05-31T01:26:23Z

by Singh, Ravinder
https://www.jove.com/t/59635
Format: Visual Materials

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

2024-05-31T01:26:23Z

by Colin, Béatrice
https://www.jove.com/t/59570
Format: Visual Materials

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

2024-05-31T01:26:23Z

by O'Sullivan, Sean J.
https://www.jove.com/t/60612
Format: Visual Materials

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

2024-05-31T01:26:23Z

by Kaur, Keerat
https://www.jove.com/t/60832
Format: Visual Materials

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

2024-05-31T01:26:23Z

by Mueller, Andreas
https://www.jove.com/t/59377
Format: Visual Materials

Manipulation of Gene Function in Mexican Cavefish

2024-05-31T01:26:23Z

by Stahl, Bethany A.
https://www.jove.com/t/59093
Format: Visual Materials

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

2024-05-31T01:26:23Z

by López-Salmerón, Vadir
https://www.jove.com/t/59394
Format: Visual Materials

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

2024-05-31T01:26:23Z

by Lee, Joonsun
https://www.jove.com/t/59202
Format: Visual Materials

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

2024-05-31T01:26:23Z

by Yu, Lina
https://www.jove.com/t/59830
Format: Visual Materials

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

2024-05-31T01:26:23Z

by Tian, Tian V.
https://www.jove.com/t/60960
Format: Visual Materials

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

2024-05-31T01:26:23Z

by Korth, Johannes
https://www.jove.com/t/59755
Format: Visual Materials

Characterizing Mutational Load and Clonal Composition of Human Blood

2024-05-31T01:26:23Z

by Huber, Axel Rosendahl
https://www.jove.com/t/59846
Format: Visual Materials

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

2024-05-31T01:26:23Z

by Gevedon, Olivia
https://www.jove.com/t/59720
Format: Visual Materials

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

2024-05-31T01:26:23Z

by Woodward, Lauren
https://www.jove.com/t/59913
Format: Visual Materials

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

2024-05-31T01:26:23Z

by Ma, Linyuan
https://www.jove.com/t/59512
Format: Visual Materials

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

2024-05-31T01:26:23Z

by Gulla, Snorre
https://www.jove.com/t/59455
Format: Visual Materials

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

2024-05-31T01:26:23Z

by Przanowski, Piotr
https://www.jove.com/t/59449
Format: Visual Materials

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

2024-05-31T01:26:23Z

by Seo, Hyun Ah
https://www.jove.com/t/59628
Format: Visual Materials

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

2024-05-31T01:26:23Z

by Kaul, Tiffany
https://www.jove.com/t/59771
Format: Visual Materials

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

2024-05-31T01:26:23Z

by Heins, Zachary J.
https://www.jove.com/t/59652
Format: Visual Materials

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

2024-05-31T01:26:23Z

by Outh-Gauer, Sophie
https://www.jove.com/t/59422
Format: Visual Materials

High-Resolution Comparison of Bacterial Conjugation Frequencies

2024-05-31T01:26:23Z

by Shintani, Masaki
https://www.jove.com/t/57812
Format: Visual Materials

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

2024-05-31T01:26:23Z

by Rickner, Hannah D.
https://www.jove.com/t/59120
Format: Visual Materials

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

2024-05-31T01:26:23Z

by Schmidt, John A.
https://www.jove.com/t/59732
Format: Visual Materials

An Assay for Quantifying Protein-RNA Binding in Bacteria

2024-05-31T01:26:23Z

by Katz, Noa
https://www.jove.com/t/59611
Format: Visual Materials

Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform

2024-05-31T01:26:23Z

by Beheshti, Afshin
https://www.jove.com/t/58447
Format: Visual Materials

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

2024-05-31T01:26:23Z

by Birnbaum, Stephanie S.L.
https://www.jove.com/t/58044
Format: Visual Materials

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

2024-05-31T01:26:23Z

by Marayati, Bahjat F.
https://www.jove.com/t/59133
Format: Visual Materials

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

2024-05-31T01:26:23Z

by Cardamone, Maria Dafne
https://www.jove.com/t/58682
Format: Visual Materials

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

2024-05-31T01:26:23Z

by Feltwell, Theresa
https://www.jove.com/t/58679
Format: Visual Materials

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

2024-05-31T01:26:23Z

by Sorlien, Erin L.
https://www.jove.com/t/56969
Format: Visual Materials

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

2024-05-31T01:26:23Z

by Guo, Xuan
https://www.jove.com/t/57889
Format: Visual Materials

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

2024-05-31T01:26:23Z

by Haupt, Amanda
https://www.jove.com/t/58130
Format: Visual Materials

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

2024-05-31T01:26:23Z

by Rankin, Carl Robert
https://www.jove.com/t/59233
Format: Visual Materials

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

2024-05-31T01:26:23Z

by Evans, Ben A.
https://www.jove.com/t/58764
Format: Visual Materials

Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi

2024-05-31T01:26:23Z

by Miyakawa, Misato O.
https://www.jove.com/t/58521
Format: Visual Materials

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

2024-05-31T01:26:23Z

by Wong, Wing H.
https://www.jove.com/t/57509
Format: Visual Materials

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

2024-05-31T01:26:23Z

by Yoshida, Yuki
https://www.jove.com/t/57615
Format: Visual Materials

Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations

2024-05-31T01:26:23Z

by Kittredge, Alec
https://www.jove.com/t/57791
Format: Visual Materials

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

2024-05-31T01:26:23Z

by Bhaskaran, Vivek
https://www.jove.com/t/60215
Format: Visual Materials

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

2024-05-31T01:26:23Z

by Memi, Fani
https://www.jove.com/t/57920
Format: Visual Materials

CRISPR Guide RNA Cloning for Mammalian Systems

2024-05-31T01:26:23Z

by Nageshwaran, Sathiji
https://www.jove.com/t/57998
Format: Visual Materials

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

2024-05-31T01:26:23Z

by Baptista, Tiago
https://www.jove.com/t/57982
Format: Visual Materials

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

2024-05-31T01:26:23Z

by Kosuta, Ceres
https://www.jove.com/t/58024
Format: Visual Materials

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

2024-05-31T01:26:23Z

by Cornwell, Adam B.
https://www.jove.com/t/57819
Format: Visual Materials

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

2024-05-31T01:26:23Z

by Krishnamani, Venkatramanan
https://www.jove.com/t/57802
Format: Visual Materials

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

2024-05-31T01:26:23Z

by Schoenfelder, Stefan
https://www.jove.com/t/57320
Format: Visual Materials

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

2024-05-31T01:26:23Z

by Saragliadis, Athanasios
https://www.jove.com/t/58267
Format: Visual Materials

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

2024-05-31T01:26:23Z

by Carleton, Julia B.
https://www.jove.com/t/57883
Format: Visual Materials

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

2024-05-31T01:26:23Z

by Moss, Caitlin E.
https://www.jove.com/t/57758
Format: Visual Materials

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

2024-05-31T01:26:23Z

by Kofoed, Rikke H.
https://www.jove.com/t/57056
Format: Visual Materials

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

2024-05-31T01:26:23Z

by Marin, Diego
https://www.jove.com/t/57442
Format: Visual Materials

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

2024-05-31T01:26:23Z

by Torres, Manon
https://www.jove.com/t/57379
Format: Visual Materials

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

2024-05-31T01:26:23Z

by Prior, Harriet
https://www.jove.com/t/57518
Format: Visual Materials

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

2024-05-31T01:26:23Z

by Siefert, Joseph C.
https://www.jove.com/t/57146
Format: Visual Materials

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

2024-05-31T01:26:23Z

by Tersey, Sarah A
https://www.jove.com/t/54838
Format: Visual Materials

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

2024-05-31T01:26:23Z

by Fourie, Nicolaas H.
https://www.jove.com/t/54693
Format: Visual Materials

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

2024-05-31T01:26:23Z

by Pathak, Rupak
https://www.jove.com/t/55162
Format: Visual Materials

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

2024-05-31T01:26:23Z

by Spokevicius, Antanas
https://www.jove.com/t/54553
Format: Visual Materials

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

2024-05-31T01:26:23Z

by Bogliotti, Yanina S.
https://www.jove.com/t/54465
Format: Visual Materials

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

2024-05-31T01:26:23Z

by Zautner, Andreas E.
https://www.jove.com/t/54165
Format: Visual Materials

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

2024-05-31T01:26:23Z

by Hölscher, Theresa
https://www.jove.com/t/54752
Format: Visual Materials

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

2024-05-31T01:26:23Z

by Skowronek, Krzysztof J.
https://www.jove.com/t/60807
Format: Visual Materials

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

2024-05-31T01:26:23Z

by Palmer, Melissa B.
https://www.jove.com/t/60736
Format: Visual Materials

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

2024-05-31T01:26:23Z

by Hobbs, Cheryl A.
https://www.jove.com/t/59955
Format: Visual Materials

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

2024-05-31T01:26:23Z

by Miller, Marisa E.
https://www.jove.com/t/55528
Format: Visual Materials

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

2024-05-31T01:26:23Z

by Diop, Soda Balla
https://www.jove.com/t/56029
Format: Visual Materials

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

2024-05-31T01:26:23Z

by Frimodt-Møller, Jakob
https://www.jove.com/t/55946
Format: Visual Materials

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

2024-05-31T01:26:23Z

by Albitar, Adam Z.
https://www.jove.com/t/55130
Format: Visual Materials

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

2024-05-31T01:26:23Z

by Subhash, Santhilal
https://www.jove.com/t/55773
Format: Visual Materials

Protocols for C-Brick DNA Standard Assembly Using Cpf1

2024-05-31T01:26:23Z

by Li, Shi-Yuan
https://www.jove.com/t/55775
Format: Visual Materials

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

2024-05-31T01:26:23Z

by Hernandez, Alfredo J.
https://www.jove.com/t/55312
Format: Visual Materials

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

2024-05-31T01:26:23Z

by Porter, Joshua R.
https://www.jove.com/t/55219
Format: Visual Materials

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

2024-05-31T01:26:23Z

by Gold, Scott E.
https://www.jove.com/t/55239
Format: Visual Materials

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

2024-05-31T01:26:23Z

by Ramlee, Muhammad Khairul
https://www.jove.com/t/55586
Format: Visual Materials

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

2024-05-31T01:26:23Z

by Amrit, Francis R. G.
https://www.jove.com/t/55473
Format: Visual Materials

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

2024-05-31T01:26:23Z

by Miousse, Isabelle R.
https://www.jove.com/t/54969
Format: Visual Materials

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

2024-05-31T01:26:23Z

by Gabrieli, Paolo
https://www.jove.com/t/54528
Format: Visual Materials

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

2024-05-31T01:26:23Z

by Bösch, Kristin
https://www.jove.com/t/54522
Format: Visual Materials

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

2024-05-31T01:26:23Z

by Samra, Nitzan
https://www.jove.com/t/54467
Format: Visual Materials

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

2024-05-31T01:26:23Z

by Liu, Po C.
https://www.jove.com/t/54426
Format: Visual Materials

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

2024-05-31T01:26:23Z

by Yeh, Szu-I
https://www.jove.com/t/54424
Format: Visual Materials

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

2024-05-31T01:26:23Z

by Dlugosz, Elizabeth M.
https://www.jove.com/t/54300
Format: Visual Materials

RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

2024-05-31T01:26:23Z

by Yoo, Aeri
https://www.jove.com/t/54280
Format: Visual Materials

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

2024-05-31T01:26:23Z

by Jeong, Haeyoung
https://www.jove.com/t/54446
Format: Visual Materials

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

2024-05-31T01:26:23Z

by Miyaguchi, Hajime
https://www.jove.com/t/54402
Format: Visual Materials

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

2024-05-31T01:26:23Z

by Yu, Ai-Qun
https://www.jove.com/t/54371
Format: Visual Materials

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

2024-05-31T01:26:23Z

by Noma, Kentaro
https://www.jove.com/t/54810
Format: Visual Materials

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

2024-05-31T01:26:23Z

by Migneault, Francis
https://www.jove.com/t/60654
Format: Visual Materials