Solid phase extraction of ibuprofen in waters with molecularly imprinted polymers prior to HPLC-DAD determination
tarafından
Ölçer, Yekta Arya, author.
Başlık
:
Solid phase extraction of ibuprofen in waters with molecularly imprinted polymers prior to HPLC-DAD determination
Yazar
:
Ölçer, Yekta Arya, author.
Yazar Ek Girişi
:
Ölçer, Yekta Arya, author.
Fiziksel Tanımlama
:
ix, 43 leaves:+ 1 computer laser optical disc.
Özet
:
Endocrine disrupting compounds (EDCs) attract great attention worldwide due to their undesired effects on human health. Ibuprofen, an example of endocrine disrupters, is a nonsteroidal anti-inflammatory drug (NSAID). In this study, highly selective molecularly imprinted polymers (MIPs) with different morphologies (as monolith and microspherical beads) were synthesized by bulk and precipitation polymerization strategies. MIPs were prepared by using acetonitrile as porogen, methacrylic acid (MAA) as monomer, trimethylolpropane trimethacrylate (TRIM) as crosslinker and the analyte, ibuprofen, as the template. MIPs revealed higher affinity to the template molecule as compared with non-imprinted polymers (NIPs). The MIP prepared by precipitation polymerization was decided to be used as the primary solid phase extraction (SPE) sorbent due to its higher binding capacity towards ibuprofen compared to the MIP prepared by bulk polymerization. Selectivity of MIP to ibuprofen was examined in the presence of structurally related compounds. In this study, a molecular imprinting solid phase extraction (MISPE) methodology was proposed for determination of ibuprofen prior to HPLC-DAD analysis. For this purpose, critical experimental parameters of MISPE method were optimized and determined as follows; solution pH of 8.0, sorbent amount of 25.0 mg for 10.0 mL of 1.0 mgL-1 working solution, sorption time of 30 min and MeOH:H2O (acetic acid, pH 3.0) ratio of 80:20 as desorption solution. The accuracy of the proposed methodology was verified with spike recovery tests for tap and drinking waters and overall recovery was found as 97.4 (± 0.3) for n=3.
Konu Başlığı
:
Extraction (Chemistry).
Solid-phase analysis.
Ibuprofen.
Endocrine disrupting chemicals.
Yazar Ek Girişi
:
Eroğlu, Ahmet Emin,
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Chemistry.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology:Chemistry.
İzmir Institute of Technology:Chemistry--Thesis (Master).
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T001521 | QD63.E88 O43 2016 | Tez Koleksiyonu |