Analysis of TNFRSF10B-As long-noncoding RNA's effects on various cancer cell properties
tarafından
Alkan, Ayşe Hale, author.
Başlık
:
Analysis of TNFRSF10B-As long-noncoding RNA's effects on various cancer cell properties
Yazar
:
Alkan, Ayşe Hale, author.
Yazar Ek Girişi
:
Alkan, Ayşe Hale, author.
Fiziksel Tanımlama
:
ix, 44 leaves: illustrarions, charts;+ 1 computer laser optical disc.
Özet
:
Long noncoding RNAs (lncRNAs) being longer than 200 nucleotides constitute a different class of RNA molecules. Several studies indicated that they have regulatory role in cellular processes including cancer development. Some of them have exclusively high expression in particular cancer types and regulate certain cancer cell properties. This renders them potential biomarker or therapeutic target in cancer. In this study, effects of a candidate lncRNA TNFRSF10B-AS and lncCAMTA1 on cancer cell properties were investigated. Candidate lncRNAs from Doxorubicin, Fas mAB, TNF-alpha and Cisplatin treated HeLa cell line were chosen and their expression level was measured in different cell lines including healthy (BEAS2B and MCF10A), metastatic (H1299 and MDA-MB- 231) and non-metastatic cell lines (A549 and MCF-7) by qPCR. From a few candidates lncCAMTA1 and TNFRSF10B-AS were selected for further analysis. qPCR results obtained from comparison of different cancer cell lines showed that their expression differs at least in one comparison of cell lines. TNFRSF10B-AS silencing decreased proliferation of HeLa cells. lncCAMTA1 was silenced or overexpressed in HeLa cells but phenotypic effect couldn’t be detected by apoptosis and cell proliferation assay. Additionally, phenotypic effect also couldn’t be observed in other cell lines when TNFRSF10B-AS was silenced.
Konu Başlığı
:
Non-coding RNA.
Cancer cells.
Yazar Ek Girişi
:
Akgül, Bünyamin,
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics--Thesis (Master).
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T002070 | QP623 .A41 2019 | Tez Koleksiyonu |