Tissue engineering is a biomedical engineering field that provides solutions to restore, maintain, improve or replace tissues or whole organs. The main goal tissue engineering is to overcome the restrictions of existing treatments that are based on organ transplantation. Cells and biomaterials can be used to form functional tissues and organs. Actually, the goal is to produce structures that resemble and mimic the real tissues. One of the useful mimicking technique is cell patterning. Cell patterning is a technique that provides cell clusters are located at a proper position for function of tissues. Some of the patterning techniques uses cell adhesion ligands, optical tweezers, acoustic tweezers, dielectrophoresis and magnetic force. In addition to the advantages of all these techniques, there are also disadvantages. However, Magnetic force-based cell patterning techniques provide excellent advantages such as low adverse effects to cell. Magnetophoresis is one of the magnetic force-based cell patterning technique that forms cell patterns without labeling cells in a short time using the principle of movement of the cells to lower magnetic field region in a paramagnetic medium. In this study, a cell patterning system was used to form cell patterns via magnetophoresis. Results showed that cell patterns were formed in different shapes in a short time and they maintained integrity even if magnetic force was removed.