Magnetic levitation of cells from bone marrov origin
tarafından
Anıl İnevi, Müge, author.
Başlık
:
Magnetic levitation of cells from bone marrov origin
Yazar
:
Anıl İnevi, Müge, author.
Yazar Ek Girişi
:
Anıl İnevi, Müge, author.
Fiziksel Tanımlama
:
xviii, 108 leaves: charts;+ 1 computer laser optical disc.
Özet
:
Magnetic levitation via negative magnetophoresis is a new label-free technology that is important in cell- and tissue-level bioengineering applications. Biofabrication applications of the technology is an area that still needs to be developed. In this doctoral thesis, 3D cellular structures with contrable size and cellular arrangement were formed and cultured with magnetic levitation using bone marrow-derived stem cells in both a miniature system that provides levitation between two magnets and a ring magnet-based large-scale system. First, a miniaturized magnetic levitation system that allows real-time imaging was produced and comprehensive protocols were described for its use for both single-cell level analysis and cell culture. With this setup, complex in situ 3D cellular aggregates were formed and their culture was maintained by levitation. Then, a new system that provides levitation on a single ring magnet was produced and used for biofabrication for the first time to overcome the reservoir volume constraint in the existing system and thus to create larger and symmetrical 3D cellular clusters. With the elimination of the upper limit in the system, the volume of the chamber was increased and the medium and biological structure transfer became easily applicable. It has been shown that this ring magnet-based magnetic levitation setup is suitable for cell culture, formation of millimeter-sized cellular structures with various cell types, and that preformed cellular structures can be combined by levitation. The low-cost and easy-to-use systems presented in this thesis have the potential to be applied in many areas such as tissue engineering and drug testing.
Konu Başlığı
:
Stem cells.
Bone marrow cells.
Permanent magnets.
Yazar Ek Girişi
:
Özçivici, Engin,
Güven, Sinan,
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Bioengineering.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology:Bioengineering.
İzmir Institute of Technology: Bioengineering--Thesis (Master).
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T002364 | QH588.S83 A59 2021 | Tez Koleksiyonu |