Investigation of the functions of candidate miRNAs in camptothecin-induced apoptosis in human cells
tarafından
Demir, Şeyda.
Başlık
:
Investigation of the functions of candidate miRNAs in camptothecin-induced apoptosis in human cells
Yazar
:
Demir, Şeyda.
Yazar Ek Girişi
:
Demir, Şeyda.
Yayın Bilgileri
:
[s.l.]: [s.n.], 2012.
Fiziksel Tanımlama
:
ix, 22 leaves. : ill. + 1 computer laser optical disc.
Özet
:
MicroRNAs are non-coding 19-25nt long, small RNAs that regulate expression of about 30% of human genes by inhibiting mRNA translation or inducing its degradation. MicroRNAs play important role in cell growth, differentiation, apoptosis. miRNAs regulate apoptosis by targeting genes involved in apoptotic pathway as a pro or anti-apoptotic genes. This study has aimed to identify whether candidate miRNAs ( miR-17* and miR-425) have a regulatory role in camptothecin induced apoptosis or not in Human cells and Hela cells that derived from cervical cancer were used as a model cell line. These candidates were selected based on deep sequencing data that showed some miRNAs differentially expressed after camptothecin treatment as compared with non-treated control group. To show candidate miRNAs whether have a role or not in regulation of camptothecin induced apoptosis, first Hela cells were transfected with candidate miRNAs then candidate miRNA over-expressed cells were treated with camptothecin eventually level of apoptosis was measured by flow cytometry and the results were evaluated by comparing miRNA over-expressed cell group with un-transfected control group. Active caspase-3 level also was measured by using flow cytometry and the data showed miR-17* and miR-425 function as pro-apoptotic regulator in camptothecin induced apoptosis in Hela cells.
Konu Başlığı
:
Apoptosis.
RNA editing.
Genetic regulation.
Yazar Ek Girişi
:
Akgül, Bünyamin.
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics.
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T001044 | QH450 .D378 2012 | Tez Koleksiyonu |