Construction of cDNA library from Hordeum marinum to indentify salt tolerance genes
tarafından
Alkaya, Naki, author.
Başlık
:
Construction of cDNA library from Hordeum marinum to indentify salt tolerance genes
Yazar
:
Alkaya, Naki, author.
Yazar Ek Girişi
:
Alkaya, Naki, author.
Fiziksel Tanımlama
:
ix, 30 leaves:+ 1 computer laser optical disc.
Özet
:
Salt is necessary for plants because of ion homeostasis; however, excess uptake of salt leads to damage in plant cells, which may even result in the death of the plant. Hordeum marinum, also known as sea barley, is a member of Poaceae family that resides in coastal areas, so it is thought that it may have a possible salt tolerance gene or genes. Therefore, this study aims to identify the genes involved in salt tolerance in Hordeum marinum by functional genomics method. After screening, seven transformant yeast colonies found and sequence analyses of these plasmids gave homology to hypothetical protein of Bipolaris oryzae. To confirm salt tolerance of this protein, salt sensitive yeast cell transfected by this candidate gene was checked in high salt concentration containing medium. Based on solid growth assay, these transgenic yeast cells could survive in 1M saline medium. Hence, it is hypothesized that Hordeum marinum and Bipolaris oryzae might have a symbiotic association. It is possible that in this association Bipolaris oryzae may play a role as endophytic fungus that might also confer salt tolerance in Hordeum marinum.
Konu Başlığı
:
Plant genetic engineering.
Plants -- Effect of salt on.
Salt-tolerant crops.
Yazar Ek Girişi
:
Karakaya, Hüseyin Çağlar
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Molecular Biology and Genetics
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics.--Thesis (Master).
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T001351 | QK753.S3 A47 2015 | Tez Koleksiyonu |