Pathogens present in the food we consume and the water we drink pose a major threat to human health. Another major health concern is the metastasis of cancer in which cancer cells spread to new areas of the body, often by way of the lymph system or bloodstream. To minimize the burden on health and economy, the detection of biomicroparticles such as pathogens or circulating cancer cells in a highly sensitive and practical manner is higly desirable. This thesis aims to develop a method to create graphene-based biosensor substrate for detection of biomicroparticles such as bacteria, viruses or mammalian cells. For this aim, graphene surface was first functionalized using a linker molecule. The effect of solvent type on functionalization was investigated via Raman spectroscopy and X-Ray spectroscopy (XPS). AntiCD2 antibodies (Ab), as a model antibody, were then conjugated to the functionalized graphene via NHS/EDC chemistry. The Ab conjugation was verified by Raman spectroscopy and XPS analyses. Finally, Jurkat cells, as model biomicroparticles, were recognized and captured by Abconjugated graphene surface, as evidenced by optical microscopy. The temperature, medium, and method for interaction of cells with graphene surfaces as well as the specificity of the Ab- functionalized graphene surface were investigated. The results overall showed the specific and efficient recognition of model cell line by Abconjugated graphene surfaces.