Calcium carbonate (CaCO3) has been used extensively as filling material in various industries in order to improve some mechanical properties of the composite materials and to reduce the product costs. There are mainly two methods for synthesizing CaCO3 crystals: chemical method and carbonization method. The carbonization method is the most appropriate method for nano calcite production. A systematic study was conducted on the synthesis of calcite in nano sizes, homogeneous size distribution, and different morphologies by employing the newly developed small penetration method. The effects of various parameters on the particle size and morphologies such as flow rates of raw materials, concentration, pipe diameter, volumes of stabilization tank and reaction chamber, length in the reaction chamber, stirring rate, and temperature were investigated. Calcite particles of about 100-150 nm were achieved to produce in homogeneous size distributions for the developed method at large scale.