Recently, the use of biomaterials with natural extracts have been rapidly increasing as an alternative to antibiotic-loaded biomaterials in wound healing applications. Cissus quadrangularis (CQ) is a natural extract which has antiinflammatory, anti-bacterial and anti-fungal activities. CQ has been used as a natural therapy in India to treat many problems such as bone repair, skin infection and wound. In this thesis study, it was aimed to prepare and characterize a bilayer wound dressing consisting of CQ extract that can mimic skin structure and to investigate extract release from the wound dressing. CQ loaded chitosan nanofibers were coated on the chitosan/POSS composite sponges with the electrospinning method as a bilayer wound dressing to treat exudate wounds. The morphology and encapsulation efficiency of the extract-loaded chitosan nanofibers are optimized to achieve controlled release of the extract. Morphologies of nanofibers were observed by scanning electron microscopy (SEM) analysis. Bilayer sponges were characterized by FT-IR, swelling, porosity, mechanical properties, antimicrobial activity, and cytotoxicity analyzes. The diameters of the nanofibers were found between 77.9±4 nm and 97.4±4 nm. The water vapor permeability of the wound dressings is between 4021 and 4609 g/m2day which is found in the appropriate range for wound healing. Bilayer dressings reached 78% to 80% cumulative release at the end of fourth day and release medium showed antimicrobial activity against E. coli and S. epidermidis. Wound dressings have not shown any toxic effects on the 3T3 cell line. Consequently, the extract loaded bilayer sponges are found as potential candidates for wound dressing.