Differentiation of human naive CD4+T cells into Th17 cell phenotype
tarafından
Akıncılar, Semih Can.
Başlık
:
Differentiation of human naive CD4+T cells into Th17 cell phenotype
Yazar
:
Akıncılar, Semih Can.
Yazar Ek Girişi
:
Akıncılar, Semih Can.
Yayın Bilgileri
:
[s.l.]: [s.n.], 2011.
Fiziksel Tanımlama
:
x, 32 leaves.: ill. + 1 computer laser optical disc.
Özet
:
Th17 cells are recently identified CD4+ T cell subset and best defined as their secretion of IL17, an inflammatory cytokine, and their role in host defense and autoimmunity. Further information on Th17 cell subset is strongly correlated with the differentiation and maintenance of these cells in culture. Although the master regulators and culture conditions in mouse Th17 differentiation are defined, the requirements and maintenance of human Th17 cell cultures remain unclear. Here, we suggest a new culture condition that maximizes IL17 expression and gives minimum IL17+IFNg+ and IL17+Foxp3+ among human studies. Our data define the doses and combinations of various cytokines that rise IL17 expression, as well as regulatory molecules in human Th17 cell differentiation. Various combinations of cytokines reveal that IL1β is the most important cytokine that primes Th17 differentiation. Also it was observed that TGFβ positively regulates Th17 differentiation in a dose-dependent manner by inhibiting IFNγ expression thus represses Th1 differentiation and impairs Treg polarization at transcription factor state by tightly regulating Foxp3. Although it was suggested that Th17 cells must express RORC2, the master regulator, a minor cell population that expresses IL17, but does not co-express RORC2 was observed.
Konu Başlığı
:
T cells.
Th1 cells.
Th2 cells.
Yazar Ek Girişi
:
Nalbant, Ayten.
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics.
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T000996 | QR185.8.T2 A315 2011 | Tez Koleksiyonu |