Tissue engineering combines the knowledge of the engineering aspects with life sciences to improve human health. Recent studies in tissue engineering have focused on investigating biocompatible scaffold materials and design. Quince seed hydrogel(QSH) has been used in traditional and modern medicine for skin wound and burn treatments, synovial lubrication, cough and asthma removal, and oral drug delivery with its antioxidant potential and biocompatible aspects. This thesis focuses on developing QSH and evaluating its potential as an injectable hydrogel in treating bone tissue defects as a totally new tissue scaffold and also as a promising tissue filling material. For this purpose, QSH scaffold optimization was carried out using various concentrations of hydrogel and crosslinkers which were glutaraldehyde(GTA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)/N-hydroxysuccinimide(NHS). Morphological and chemical analysis of QSH was done using SEM, FTIR, AFM, and protein adsorption test. Thus, porosity, swelling ratio, degradation rate and surface characteristics were evaluated. NIH-3T3 and SaOS-2 cell lines were utilized for 3D cell culture formation. Afterward, 3D spheroids were analyzed for cell viability and proliferation by using AlamarBlue and LiveDead assays, and also cell imaging technics. Results showed that QSH scaffolds did not show any cytotoxic effect on NIH-3T3 and SaOS-2 cells. The optimum results were achieved with 2mg/mL of QSH and 0.03M GTA concentrations; where 76.59μm average pore size, 56.8 fold water holding capacity and at least 80% cell viability was observed. Therefore, it was concluded that QSH has a high potential to promote tissue engineering applications with its injectable texture as a filling material.