Development of natural compound-loaded nanofibers by electrospinning
tarafından
 
Balta, Ali Bora.

Başlık
Development of natural compound-loaded nanofibers by electrospinning

Yazar
Balta, Ali Bora.

Yazar Ek Girişi
Balta, Ali Bora.

Yayın Bilgileri
[s.l.]: [s.n.], 2010.

Fiziksel Tanımlama
xiii, 137 leaves. : ill. + 1 computer laser optical disc.

Özet
In this study, the crude silk which is obtained from silkworm was turned into a silk solution after a serial procedure. Then, regenerated silk (foam) was obtained. The regenerated silk was dissolved in formic acid and polymer solution was prepared. After, nanofibers were produced by electrospinning. On the other hand, the content and antimicrobial activities of some plants were analyzed. Then all three forms of silk was absorbed with the olive leaf extract and extract desorption tests were done. As the first step of the study, the analyses of extracts which were obtained from four different plants were done. For this, total phenolic content and antioxidant capacity were found. In addition, minimum inhibition concentration (MIC) test and disc diffusion test were made for all extracts to Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa bacteria and Candida albicans fungi in order to determine their antimicrobial activity. While producing nanofibers from silk polymer with electrospinning method, different parameters such as concentration, voltage and distance were examined. Morphological characterization of nanofibers was done by scanning electron microscope (SEM). According to the results, the nanofiber with an optimum value which has a suitable diameter and structure was selected. With this nanofiber, the absorption and desorption tests of natural compound were made. The results were obtained by High pressure liquid chromatography (HPLC). Same adsorption and desorption tests were done also with the microfiber silk and regenerated silk (foam). As a result, it was shown by the controlled experiments that nanofibers were better for adsorption and desorption of natural compound when compared to microfiber silk and regenerated silk. In conclusion, nano-sized silk fibroin structures can be adsorbed with natural compounds in order to gain functionality. Using this kind of biofunctional products as medical textile and wound dressing material will be more advantageous when compared to current wound dressing materials.

Konu Başlığı
Textile Fibers -- Testing -- Laboratory manuals.
 
Silk -- Testing.
 
Nanofibers.
 
Electrospinning.

Yazar Ek Girişi
Bayraktar, Oğuz.

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology and Bioengineering.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology:Biotechnology and Bioengineering.
 
İzmir Institute of Technology:Biotechnology and Bioengineering--Thesis (Master).

Elektronik Erişim
Access to Electronic Version.


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT000216TS1540 .B19 2010Tez Koleksiyonu