Utilization of endophytic fungi and their enzymes for transformation to obtain bioactive compounds
Küçüksolak, Melis, author.

Utilization of endophytic fungi and their enzymes for transformation to obtain bioactive compounds

Küçüksolak, Melis, author.

Yazar Ek Girişi
Küçüksolak, Melis, author.

Fiziksel Tanımlama
xxxvi, 207 leaves: charts;+ 1 computer laser optical disc.

Biotransformation is a chemical reaction performed by biological systems or their components to modify molecules and has many applications in the pharmaceutical industry. In our previous project, biotransformation studies were carried out on Astragalus cycloartanes using endophytic fungi isolated from the tissues of Astragalus species, and the effects of the metabolites on telomerase activation were investigated. Among the isolated endophytic fungi, Alternaria eureka and Camarosporium laburnicola were identified as potent biocatalysts in developing molecule libraries and producing potent telomerase activators. This thesis aims to demonstrate the potential use of endophytic fungi in the production of bioactive metabolites. In the first part, biotransformation studies were performed on cyclocephagenol, a novel cycloartane-type sapogenin with tetrahydropyran unit, using Alternaria eureka and Camarosporium laburnicola, and twenty-eight new metabolites were obtained. According to the results of bioactivity studies, Alternaria eureka and Camarosporium laburnicola were found to be responsible for producing potent neuroprotective agents and potent telomerase activators, respectively. In parallel, biotransformation conditions were statistically optimized to afford potential telomerase activators, discovered in our previous studies (E-CG-01, E-AG-01 and E-AG-02). For this, nine parameters were screened by Plackett-Burman Design, and three significant parameters were optimized using Central Composite Design. As a result, production yields were increased by 1.95-fold for E-CG-01, 70-fold for E-AG-01, and 19-fold for E-AG-02. In the last part, the partial purification and characterization of alcohol dehydrogenase, Baeyer-Villiger monooxygenase, and lactone hydrolase enzymes, which were catalyzing the modifications in the production of telomerase activators, from Camarosporium laburnicola were performed.

Konu Başlığı
Bioactive compounds
Biotransformation (Metabolism)
Endophytic fungi -- Biotechnology
Astragalus (Plants)

Yazar Ek Girişi
Bedir, Erdal,
Sağ, Duygu,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Bioengineering.

Tek Biçim Eser Adı
Thesis (Doctoral)--İzmir Institute of Technology:Bioengineering.
İzmir Institute of Technology:Bioengineering --Thesis (Doctoral).

Elektronik Erişim
Access to Electronic Versiyon.

LibraryMateryal TürüDemirbaş NumarasıYer Numarası
IYTE LibraryTezT002681QP517.B44 K95 2022 V.1
IYTE LibraryTezT002682QP517.B44 K95 2022 V.2