Fungal biotransformation of novel 20(27)-octanor cycloastragenol and biological activities of the purified metabolites
tarafından
 
Duman, Seda, author.

Başlık
Fungal biotransformation of novel 20(27)-octanor cycloastragenol and biological activities of the purified metabolites

Yazar
Duman, Seda, author.

Yazar Ek Girişi
Duman, Seda, author.

Fiziksel Tanımlama
xvii, 163 leaves: color illustraltions, charts;+ 1 computer laser optical disc.

Özet
Biotransformation is the chemical modifications performed by enzymes or living organisms. The difficulty and high cost of enzyme isolation and purification makes it more advantageous to use whole cell systems as biocatalysts. Microbial biocatalysts are particularly interesting in the modification of complex molecules such as steroids and triterpenoids, which can catalyze stereo- and regio- selective reactions that are difficult or impossible to perform with chemical reactions. Specially, ability of endophytic microorganisms to produce specific enzymes for adaptation to their environment by tolerating toxic defense metabolites, makes them interesting for biotransformation studies. Telomeres are nucleotide structures located at the end of chromatids shortening with each cell division. Telomerase is a reverse transcriptase enzyme, and it helps to replenish telomere ends that are truncated by aging and stress factors. The telomerase activators (TA) with their potentials are suggested encouraging agents for healthy aging, and they are projected to generate a huge market in the future. Cycloastragenol is the only natural product in the sector marketed as a potent telomerase activator. In this study, by using endophytic fungi, the biotransformation studies were performed on 20(27)-octanor cycloastragenol. As a result, 14 biotransformation products, were isolated by chromatographic studies, and the structures of the metabolites were established by spectral methods. Based on the literature survey, 13 compounds turned out to be new for nature. Seven metabolites were screened for telomerase activation. In these screenings, metabolites showed telomerase activation ranging from 5.43 to 12.36 fold at doses ranging from 0.1 to 30 nM compared to the control cells treated with DMSO.

Konu Başlığı
Biotransformation (Metabolism)
 
Fungi -- Biotechnology.

Yazar Ek Girişi
Bedir, Erdal,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Biotechnology.
 
İzmir Institute of Technology: Biotechnology--Thesis (Master).

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer Numarası
IYTE LibraryTezT002034TP248.27.F86 D88 2019