The effects of engineered silica nanoparticles on the cellular behaviours of human hepatocellular carcinoma cell lines
Tüncel Çerik, Özge, author.

The effects of engineered silica nanoparticles on the cellular behaviours of human hepatocellular carcinoma cell lines

Tüncel Çerik, Özge, author.

Yazar Ek Girişi
Tüncel Çerik, Özge, author.

Fiziksel Tanımlama
xii, 85 leaves: color illustrarions, charts;+ 1 computer laser optical disc.

Physicochemical properties of the silica nanoparticles have vital roles in determining the physiological behaviours of the cells. Applications of nanoparticle treatments have some outcomes as a response of the cells in living systems as mitochondrial disruption, oxidative stress, reactive oxidative species (ROS) generation, altered cell cycle regulation and DNA damage. In this study 10 and 100 nm sized SiNPs were prepared and physicochemically characterized in the second part. Well characterized silica nanoparticles were used to assess the cytotoxicity and genotoxicity of the hepatocellular carcinoma cell lines as HuH-7 and SK-HEP-1 and lymphocytes. The cell cycle analysis was performed for engineered SiNPs to elucidate the DNA damage in the third part. In the fourth part mitochondrial responses of the cells were determined by real time confocal microscopy at single cell level. An image analysis method for evaluating the cellular responses by mitochondrial staining was developed. DCF stained cells were analyzed in order to assess the production of ROS in the cells. Localization of the SiNPs were determined by lysosomal and mitochondrial staining. Pearson correlation coefficients of the images were used for evaluating the colocalization of organelles with SiNPs. Lastly, diffusion coefficients of the SiNPs in the cells were determined by quantitative confocal microscopy. The SiNPs were found as non-toxic up to 200 μg/ml for 5 days. The SiNPs did not induce the formation of micronuclei in lymphocytes. The SiNPs were not cause an arrest in cell cycle progression. Mitochondrial potentials were not changed after SiNP exposure as well. They were mostly internalized at 30 minutes in both cell line in lysosomal parts without increasing ROS in the cells. It can be concluded that the SiNPs can be safely used for targeted delivery of organic compounds, biological molecules or drugs in medicine, and may be utilized as a probe system in biological studies.

Konu Başlığı
Chemistry, Physical and theoretical.
Liver -- Cancer.

Yazar Ek Girişi
Özçelik, Serdar,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Bioengineering.

Tek Biçim Eser Adı
Thesis (Doctoral)--İzmir Institute of Technology: Bioengineering.
İzmir Institute of Technology: Bioengineering--Thesis (Doctoral)

Elektronik Erişim
Access to Electronic Versiyon.

KütüphaneMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTETezT001810TA164 .T92 2018Tez Koleksiyonu