Development of plasmonic nanostructures for photothermal therapy of prostate and breast cancer
Tomak, Aysel, author.

Development of plasmonic nanostructures for photothermal therapy of prostate and breast cancer

Tomak, Aysel, author.

Yazar Ek Girişi
Tomak, Aysel, author.

Fiziksel Tanımlama
xv, 124 leaves: illustrarions, charts;+ 1 computer laser optical disc.

The aim of this thesis is to synthesize gold nanorods (AuNRs) and lipid-stabilized nanobubbles containing AuNRs and investigate the potential of these plasmonic nanostructures as photothermal therapy agents for breast and prostate cancer through in vitro cell culture experiments. For this aim, firstly, AuNRs were synthesized at varying aspect ratios (ARs) and characterized via several techniques including UV-Vis/NIR spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), inductively coupled plasma-mass spectroscopy (ICP-MS), electrophoretic light scattering (ELS) and X-ray photoelectron spectroscopy (XPS). The surface of AuNRs was modified with a biocompatible polymer, poly(ethylene glycol) (PEG), via ligand exchange method. Cytotoxicity, cell uptake and photothermal effects of AuNRs were investigated via in vitro cell culture experiments using human prostate cancer (DU 145) and epithelial (RWPE-1), breast cancer (MCF7) and epithelial (MCF 10A) cell lines. It was concluded that AuNRs (AR=4.0) were superior than AuNRs (AR=7.0) in terms of cell viability and photothermal effect. Separately, a non-commercial antibody (Ab) targeting a specific sialic acid derivative on the plasma membrane of DU 145 and MCF7 cancer cells was conjugated to AuNRs. Conjugations were characterized with the same techniques and investigated via in vitro cytotoxicity and cell uptake experiments. The Ab-conjugated AuNRs displayed the capability of selective targeting prostate cancer cells. Additionally, lipid-stabilized AuNRs and lipid-stabilized nanobubbles containing AuNRs (AuNBs) were synthesized for the first time and characterized using UV-Vis/NIR spectroscopy, SEM, ICP-MS and ELS techniques. Lipid-stabilized AuNRs were successfully synthesized using varying lipid mixtures instead of cationic, toxic surfactant. Separately, AuNBs were synthesized by combining PEG modified AuNRs with DPPC: DSPE-PEG lipid film under sonication and gas stream. AuNBs showed the same or significantly lower toxicity depending on the cell types and the same photothermal effect with respect to AuNRs (AR=4.0) upon irradiation under laser at 808 nm.

Konu Başlığı
Nanostructured materials -- Therapeutic use.
Cancer -- Diagnosis.
Breast -- Cancer.
Prostate -- Cancer.
Cancer -- Treatment.
Photothermal spectroscopy.

Yazar Ek Girişi
Bulmuş, Volga

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Materials Science and Engineering.

Tek Biçim Eser Adı
Thesis (Doctoral)--İzmir Institute of Technology: Materials Science and Engineering.
İzmir Institute of Technology: Materials Science and Engineering--Thesis (Doctoral).

Elektronik Erişim
Access to Electronic Versiyon.

LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT002073R857.N34 T65 2019Tez Koleksiyonu