Cloning of novel sericin like proteins and optimization of their expression
tarafından
 
Gürerk, Gamze, author.

Başlık
Cloning of novel sericin like proteins and optimization of their expression

Yazar
Gürerk, Gamze, author.

Yazar Ek Girişi
Gürerk, Gamze, author.

Fiziksel Tanımlama
x, 74 leaves: charts;+ 1 computer laser optical disc.

Özet
Sericin is a protein that helps fibroin fibers link together to create the cocoon, and it forms silk with fibroin. Fibroin is used in textile production as well as in biomaterial applications. Sericin has essential biocompatibility, biodegradability, cryoprotection, antioxidant properties, and a multi-component structure. Thanks to these properties, its use in biomaterial and biomedical fields is expanding and also used in cosmetic research and wound healing, and drug delivery thanks to its high fiber structure. Sericin can be obtained from silkworm cocoons by degumming method but changes the structure of the obtained protein since the protein is exposed to high temperatures. Sericin produced in different batches is obtained in different quantities and these create inconsistencies in the quality of the biomaterials obtained from the sericin, limiting the use of the sericin as a biomaterial. Besides, obtaining protein by recombinant production provides the advantage that the repetitive chain length can adjust as desired and the protein can be standardized. In this thesis, recombinantly generated and optimized a novel sericin-like protein (Ser-12mer) with the native sericin sequence encoding twelve repeats of recombinantly conserved 38 amino acid motifs in Escherichia coli and characterized its structural properties. In addition, the effects of induction cell density and cell culture media on the expression of the previously produced sericin-like protein (Ser-4mer) was investigated and its expression and concentration were increased by optimization. Recombinant production of a sericin-like protein will provide an understanding of the sequence-structure relationships and significantly expand their applications as biomaterials.

Konu Başlığı
Recombinant proteins
 
Proteins -- Biotechnology
 
Recombinant DNA
 
Sericin

Yazar Ek Girişi
Sürmeli Eraltuğ, Nur Başak,
 
Şanlı Mohamed, Gülşah,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Biotechnology.
 
İzmir Institute of Technology: Biotechnology--Thesis (Master).

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer Numarası
IYTE LibraryTezT002485TP248.65.P76 G97 2022