Identification of genes that play roles in boron metabolism
tarafından
 
Kaya, Alaattin.

Başlık
Identification of genes that play roles in boron metabolism

Yazar
Kaya, Alaattin.

Yazar Ek Girişi
Kaya, Alaattin.

Yayın Bilgileri
[s.l.]: [s.n.], 2008

Fiziksel Tanımlama
ix, 33 leaves.: ill.+ 1 computer laser optical disk.

Özet
Boron, is an essential microelement that plays a role in plant and animal development. However, an excess amount of boron is toxic for both types of organisms.The yeast Saccharomyces cerevisiae is an ideal model organism for describing the mechanism and regulation of metal ion transport in eukaryotes. We screened a yeast genomic DNA library to find genes that confer boron resistance to wild-type cells.Thirty transformants were isolated that were able to grow in the presence of a toxic amount of boron and all of them contained the multidrug efflux transporter gene ATR1 (YML116w) in the expression cassette. Our subsequent analysis revealed that ATR1 deletion mutants (.atr1) were sensitive to boron treatment and hyper-accumulated boron inside cells, whereas wild-type cells overexpressing the ATR1 gene were resistant to boron and hypoaccumulated boron in cells. We also analysed the global gene expression pattern in response to boron treatment and found that expression of ATR1 is upregulated along with many other transporter and amino acid biosynthesis genes. Our data suggest that the ATR1 gene functions as a boron efflux pump and is reqired for boron tolerance in yeast cells.

Konu Başlığı
Boron.
 
Plants, Effect of boron on.
 
Yeast -- Biotechnology.

Yazar Ek Girişi
Koç, Ahmet.

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology and Bioengineering.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Biotechnology and Bioengineering.
 
İzmir Institute of Technology: Biotechnology and Bioengineering--Thesis (Master).

Elektronik Erişim
Access to Electronic Version.


LibraryMateryal TürüDemirbaş NumarasıYer Numarası
IYTE LibraryTezT000675TP245.B6 K233 2008