Single-photon sources are essential components for several applications in the field of quantum information technologies, such as quantum cryptology and quantum computation. To this aim, efficient generation and detection of single-photons are the crucial to be achieved. Among single-photon sources that are extensively studied in the literature, defect centers in solid are very promising due to their room temperature operation and their stability. The aim of this thesis is to generate single photons at room temperature and control their optical properties by nanostructures. Single-photon emission from TMDCs originates from localized weakly bound excitons at cryogenic temperatures due to their small exciton binding energies. However, room temperature SP emission from WS2 can be obtained by creatingWO3 defects. In our study, room temperature emission from defects in WO3 was investigated. Density functional theory calculations showed that the source of the emission can be oxygen defects. Additionally, the emission was brightened by plasmonic gold nanoparticles. Furthermore, defects in two-dimensional (2D) hexagonal boron nitride (hBN) is offered as an efficient room temperature SPS. HBN is a wide bandgap 2D material, in which defect centers create discrete energy level to generate single photons. In our study, reversible single-photon emission control from defects in hBN was demonstrated by Förster-like resonance energy transfer between the single-photon emitter and a graphene layer. To this aim an ionic liquid based device structure was used.