Molecular investigation of pathogenic bacteria in the presence of phenolic acids
Özdemir, Özgün Öykü, author.

Molecular investigation of pathogenic bacteria in the presence of phenolic acids

Özdemir, Özgün Öykü, author.

Yazar Ek Girişi
Özdemir, Özgün Öykü, author.

Fiziksel Tanımlama
xx, 215 leaves: color illustrarions, charts;+ 1 computer laser optical disc.

Pathogenic bacteria, including P. aeruginosa, are serious threats for human health with their antibiotic resistance and virulence factors. Since phenolic acids, secondary metabolites of plants, can be good candidates as antimicrobial agents, their mode of action should be investigated. Proteomics is one of the main approaches for elucidating the mode of action of such compounds. In addition, in order to enhance the antimicrobial effects and stabilities of phenolics, they can be encapsulated into nanoparticles. The nanoparticles can be produced from chitosan and alginate which are biocompatible polymers. In this study, the antimicrobial effects of 3-HPAA and 4-HBA were presented with MIC values of 2.1 and 1.9 mg/ml, respectively. The bacteriocidal effects of them were also shown as 2.3 mg/ml for 3-HPAA and 2.1 mg/ml for 4-HBA. The morphological changes of bacteria were determined after phenolic acid exposure via SEM. The LC-ESI-MS/MS technique was used to show changes in the protein profile of bacteria arose from antimicrobial effects. Both phenolic exposures resulted in various protein changes especially in membrane-related proteins as well as ribosome and protein synthesis related-proteins. In addition, they caused serious oxidative stress depending on the protein profile changes related to redox proteins. Alginate-chitosan nanoparticles resulted increased antimicrobial effects of the phenolics which were produced, characterized and tested on various pathogenic bacteria via agar diffussion and spectrophotometric measurements. Hence, free or encapsulated forms of phenolic acids were demonstrated as effective antimicrobial agents and based on proteomic results, the effect of phenolic acids may be multi targetted.

Konu Başlığı
Phenolic acids.
Pathogenic bacteria.
Anti-infective agents.

Yazar Ek Girişi
Soyer Dönmez, Ferda

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Molecular Biology and Genetics.

Tek Biçim Eser Adı
Thesis (Doctoral)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics.--Thesis (Doctoral).

Elektronik Erişim
Access to Electronic Versiyon.

KütüphaneMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTETezT002019QH506 .O99 2019Tez Koleksiyonu