Expression of aquaporin 1, 3 and 4 in T cell activation and apoptosis
Gelmez, Ayşe Bengisu, author.

Expression of aquaporin 1, 3 and 4 in T cell activation and apoptosis

Gelmez, Ayşe Bengisu, author.

Yazar Ek Girişi
Gelmez, Ayşe Bengisu, author.

Fiziksel Tanımlama
ix, 43 leaves: illustrarions, charts;+ 1 computer laser optical disc.

Aquaporins (AQPs) are membrane proteins responsible for transporting water, some gases and small solutes such as CO2 and glycerol. Until now, it has been shown that AQP1, 3 and 5 expressed in both B and T lymphocytes of mice, regulate cell volume. However, aquaporin expression involved in activation, proliferation, and differentiation as well as apoptosis of T cells are not well known yet. The goal of this study is to detect the expression level of AQP1, AQP3, and AQP4 in activated and apoptotic T cells. In order to do that, two types of T cells cultured in both condition were utilized. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from Human peripheral blood drawn from healthy donors by ficoll density gradient Centrifugation method. Naive CD4+ T cells were sorted from PBMC. The stimulants generating Th17 were chosen for activation and differentiation of naïve CD4 T cells. Jurkat cell line as a second cell type were activated by PMA/Ionomycin as well as treated by camptothecin for apoptotic processing. Th17 and Jurkat cell cultures were analysed by flow cytometry to measure the rate of both activation and apoptosis. Western Blot was performed to identify expression of AQP 1, 3 and 4. We found a significance between increased expression level of AQP1, 3, and 4 in activated T cells as well as decreased expression level of each three AQPs in apoptotic T cell populations. According to our findings, tested aquaporin proteins may play roles in T cell activation, differentiation, and apoptosis. The scientific significance of this research is that it can fill the gaps about these three functional processes of T cells. Besides, all findings can contribute to treatment of many autoimmune disease like MS which Th17 cells involve in pathogeny.

Konu Başlığı
T cells -- Immunology.

Yazar Ek Girişi
Nalbant Aldanmaz, Ayten,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Molecular Biology and Genetics.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics (Master).

Elektronik Erişim
Access to Electronic Versiyon.

KütüphaneMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTETezT001735QR185.8.T2 G31 2018Tez Koleksiyonu