Proteomic studies and its application to biological samples using mass spectrometry
tarafından
 
Güray, Melda Zeynep, author.

Başlık
Proteomic studies and its application to biological samples using mass spectrometry

Yazar
Güray, Melda Zeynep, author.

Yazar Ek Girişi
Güray, Melda Zeynep, author.

Fiziksel Tanımlama
xiv, 162 leaves: illustrarions, charts;+ 1 computer laser optical disc.

Özet
Mass spectrometry (MS) is a powerful analytical tool with its application in the field of biological sciences for identification of proteins, defining post-translational modifications, studying protein expression and protein interactions. This thesis presents MS analyses of proteins for defining modifications observed during sample preparation and identification of proteins isolated from clinical samples and microorganisms. The first part of the thesis includes proteomic analysis of antimony resistant L. tropica. The results clearly indicated that the generation of antimony resistance by parasites, either in host organism or in vitro, causes alteration of protein expression levels, and the mechanism of antimony resistance in host organism and in vitro conditions follow different strategies. In the second part of the study, proteomic analysis of Bence Jones proteins isolated from urine of multiple myeloma patients was performed. Gel electrophoresis and MS analysis revealed that the proteins from different patients with different nephrotoxicity have different tendencies to form multimeric structures and contained different type of light chain. In the third part, it was shown that precipitation of proteins in acetone causes +98 u mass artifacts on proteins when analyzed by MS. The parameters affecting the formation of modification was studied and it was revealed that this modification is dependent on solution pH, incubation time and temperature. In the last part, aspartic acid and glutamic acid containing synthetic peptides were shown to be methylated upon incubation in acidified methanol solution. MS analysis revealed that the reaction is dependent on temperature and time and is affected by the type of acid included in methanol solution. All in all, this thesis provides a comprehensive study of proteins by mass spectrometry for identification of proteins from different sources, as well as defining protein modifications observed as artifacts during sample handling in proteomic workflows.

Konu Başlığı
Biochemistry.
 
Proteins.
 
Proteomics.
 
Mass spectrometry.
 
Post-translational modification.

Yazar Ek Girişi
Yalçın, Talat,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Chemistry.

Tek Biçim Eser Adı
Thesis (Doctoral)--İzmir Institute of Technology: Chemistry.
 
İzmir Institute of Technology: Chemistry--Thesis (Doctoral).

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT001669QD415 .G97 2017Tez Koleksiyonu