Just as any other interior, lighting quality of exhibition spaces need to be examined to enhance visual quality and comfort. Exhibition lighting is already a chaotic process with many quantitative and qualitative parameters, their relation with each other and concerns of multi-disciplines. Consequently, the impacts and the potentials of subjective appreciation, daylight, user perception and behavior, new developments and the relation between the parameters are often disregarded. In this thesis, a comprehensive study is conducted to understand the impact of lighting type, color temperature, room and exhibition parameters on navigation and impressions. A set of three exhibition spaces with various room and lighting conditions were modelled virtually, to be evaluated in a three-part questionnaire. A total of 90 participants are selected equally from three profession groups which are architects, visitors and artists. Their movement through the exhibition, preferences and impressions are analyzed with various statistical analysis methods. Results show that there are some distinctive preferences between occupation groups. In the first part, it can be seen that navigation choices changes with the lighting type as the movement towards daylight increases in transition areas and the end. Generally, daylight is preferred for sculpture while artificial light is preferred for paintings. In the second and third part, it was found out that lighting type is the major factor against color temperature in preference and impressions. The best setting is picked as single spotlight with neutral color temperature. Warm ambient lighting is not received well. Ultimately, lighting preferences and perception change with different room and exhibition conditions.