Plum is an economically important and popular fruit in Turkey, which ranks in sixth place in world plum production. This fruit is attractive to consumers with its beautiful scent and its delicious taste. In addition, it is an important plant species with its wealthy mineral, vitamin and phytochemical content. The genus Prunus is classified into three groups: the European, the Asian and the American plums. P. cerasifera, which was used in this study, belongs to the European group. P. cerasifera (2n=2x=16) is a Turkish plum drupe species. Since there are many subspecies, it can grow naturally in many parts of Turkey. The genetic diversity and population structure of P. cerasifera have not been studied using molecular techniques. Here, we studied the genetic diversity of 66 P. cerasifera accessions collected from Turkey at the molecular level using 47 sequence related amplified polymorphism (SRAP) primer combinations. The SRAP marker combinations showed reasonable polymorphism. A dendrogram was constructed using the Dice coefficient and unweighted neighbor joining algorithm. The dendrogram revealed three groups and the similarity between accessions ranged from 0.04 to 0.66 with an average dissimilarity of 0.37. Population structure analysis identified three subpopulations (K=3). This is the first genetic diversity analysis of P. cerasifera using SRAP markers. Genetic diversity and population structure data can be useful for determining breeding strategies in P. cerasifera accessions.