Development of microfluidic devices for investigating small molecule induced chemotaxis dendritic cells
Khurram, Muhammad Maaz, author.

Development of microfluidic devices for investigating small molecule induced chemotaxis dendritic cells

Khurram, Muhammad Maaz, author.

Yazar Ek Girişi
Khurram, Muhammad Maaz, author.

Fiziksel Tanımlama
xiii, 73 leaves: illustrarions, charts; 29 cm + 1 computer laser optical disc.

Microfluidics is the core branch of science and technology in which interdisciplinary research is conducted with a low amount of samples in microchannels ranging from 10-100 μm. The main objective of this thesis is to design and fabricate a chemotaxis microfluidic device (CMD) from the poly-methyl methacrylate (PMMA) substrate to analyze the immune cell behavior against cancer cells. The patterns of the three-layered CMD were generated using laser ablation. During the fabrication, Power (P) and Speed (S) values were varied to determine the optimal P-S combination. Then, the structural properties of microfluidic channels in the CMD were examined via microscope. The mechanical properties and liquid handling abilities of CMDs were also investigated through tensile and leakage tests, respectively. Moreover, cell viability of DC2.4 dendritic cells (DCs) and B16-F10 murine melanoma (B16-F10) cells in CMDs sterilized through either autoclaving or UV treatment were determined to test the suitability of CMDs via Live/Dead Assay. The highest cell viability for DCs and B16- F10 was obtained in autoclaved CMDs. For the maturation of DCs before seeding into CMD, DCs were stimulated with lipopolysaccharide (LPS) and Astragaloside VII (AST-VII) at various concentrations. While the cytotoxicity of LPS and AST-VII were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, the expression levels of specific chemokine receptors were also analyzed through flow cytometry. Lastly, stimulated DCs and B16-F10 were simultaneously cultured in the CMD, and the migratory behavior of DCs against B16-F10 was time-dependently studied. Consequently, CMD that provided cost-effective and rapid analysis of intercellular interactions was successfully developed.

Konu Başlığı
Microfluidic devices
Dendritic cells

Yazar Ek Girişi
Bedir, Erdal,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology and Bioengineering

Tek Biçim Eser Adı
Thesis (Master)-- İzmir Institute of Technology: Biotechnology and Bioengineering
İzmir Institute of Technology: Biotechnology and Bioengineering (Master).

Elektronik Erişim
Access to Electronic Versiyon.

LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT002855TJ853.4.M53 K45 2023Tez Koleksiyonu