Molecular investigation of P. aeruginosa in the presence of 4-HBA
tarafından
 
Ekenel, Nil Hazal, author.

Başlık
Molecular investigation of P. aeruginosa in the presence of 4-HBA

Yazar
Ekenel, Nil Hazal, author.

Yazar Ek Girişi
Ekenel, Nil Hazal, author.

Fiziksel Tanımlama
xii, 151 leaves: charts;+ 1 computer laser optical disc.

Özet
The escalating threat to human health posed by bacterial pathogens is increasingly attributed to the growing prevalence and distribution of antibiotic-resistant bacteria. In response to antibiotics, microorganisms have developed resistance mechanisms to elude and survive the impacts of these drugs. Phenolic acids have emerged as potent candidates in the battle against bacterial infections due to their unique property of not inducing resistance. This study focuses explicitly on 4-hydroxybenzoic acid (4-HBA), a type of phenolic acid, as an effective antimicrobial agent. Proteomics research has become an indispensable tool in the fight against antimicrobial resistance. Pseudomonas aeruginosa, a bacterium capable of existing in both planktonic and biofilm states and known to cause numerous human diseases, is of particular significance in this context. Furthermore, the study explores the molecular aspects of P. aeruginosa when exposed to 4-HBA through proteomic analysis, revealing a significant impact on protein biosynthesis as a predominantly affected function. Additionally, P. aeruginosa, the study investigates the effects of phenolic acid and antibiotic-loaded bone cements on Methicillin-sensitive Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, and P. aeruginosa. Remarkably, 20 percent inhibition rate is achieved after 48 hours of treatment. Moreover, the study examines biofilm produced by P. aeruginosa in the presence of 4-HBA, utilizing both the Crystal Violet assay and Scanning Electron Microscopy. Significantly, the biofilm formation is observed to be disrupted by these methodologies. Overall, this study underscores the effectiveness of 4-HBA as antimicrobial compound against diverse range of pathogens, as demonstrated through both phenotypic and proteomic approaches. Keywords: Antimicrobial resistance, Pseudomonas aeruginosa, 4-HBA, Proteomics, Bone cement.

Konu Başlığı
Pseudomonas aeruginosa.
 
Pseudomonas aeruginosa infections.
 
Anti-infective agents.
 
Proteomics
 
Bone cements

Yazar Ek Girişi
Soyer Dönmez, Ferda,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Molekular Biology and Genetics.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology:Molecular Biology and Genetics.
 
İzmir Institute of Technology: Molekular Biology and Genetics --Thesis (Master)

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT002820QR82.P78 E367 2023Tez Koleksiyonu