Design of localized surface plasmon resonance (LSPR) based biosensor for detecting a potential cancer biomarker
tarafından
Söylemez, Cansu, author.
Başlık
:
Design of localized surface plasmon resonance (LSPR) based biosensor for detecting a potential cancer biomarker
Yazar
:
Söylemez, Cansu, author.
Yazar Ek Girişi
:
Söylemez, Cansu, author.
Fiziksel Tanımlama
:
x, 50 leaves: charts;+ 1 computer laser optical disc.
Özet
:
Conventional methods for detection of cancer are invasive, expensive and not suitable for early diagnosis. Therefore, demand for simple, sensitive and rapid biosensors for detection of cancer have been enormous. Gold nanorods (GNRs) have been ideal materials for utilization in biosensors because of their exceptional optical properties. Localized surface plasmon resonance (LSPR) which is created on GNR surface can be used for the development of label-free and sensitive biosensor systems. LSPR responds to changes in the refractive index of the surroundings and this change can be observed as the shift in the maximum absorption wavelengths. In this thesis, an LSPR based GNR biosensor was developed for sensitive detection of a sialic acid as a potential cancer biomarker. For this purpose, GNRs were synthesized at around 40-50 nm in length. Afterwards, glass surfaces were coated with GNRs and functionalized with self-assembling molecules. Specific monoclonal antibodies(Ab) were conjugated to the surface. The surface modifications were characterized via contact angle, scanning electron microscope, Fourier transform infrared spectroscopy and zeta potential. Ab-functionalized glass surfaces were used to quantitatively detect specific molecular bindings via LSPR. The sensitivity of the biosensor was determined as 281 RIU/nm. The detection limit in PBS was 1 nM, while in serum it was found to be as 10 nM because of the high protein content of serum. Control experiments showed that the developed biosensor chip was selective. The proposed system is promising for early diagnosis of cancer since it can detect a potential cancer biomarker at concentrations as low as nanomolar level.
Konu Başlığı
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Biosensors.
Surface plasmon resonance
Gold -- Metallurgy.
Yazar Ek Girişi
:
Bulmuş Zareie, Esma Volga,
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Bioengineering.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology: Bioengineering.
İzmir Institute of Technology: Bioengineering--Thesis (Master).
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T002195 | R857.B54 S73 2020 | Tez Koleksiyonu |