Production and isolation of fungal chitosan by submerged fermentation
tarafından
 
Alper, Seda.

Başlık
Production and isolation of fungal chitosan by submerged fermentation

Yazar
Alper, Seda.

Yazar Ek Girişi
Alper, Seda.

Yayın Bilgileri
[s.l.]: [s.n.], 2003.

Fiziksel Tanımlama
xi, 74 leaves.: ill.+ 1 computer laser optical disc.

Özet
Chitosan is the N-deacetylated derivative of chitin which is the supporting material of crustaceans and insects. Chitosan together with chitin are recommended as suitable functional materials because of their excellent biocompatibility, biodegradability, non-toxicity and adsorption properties and can be used in agriculture, biotechnology and food industry. Although chitosan is produced by chemical deacetylation of chitin molecule, it is also a natural component of cell walls of fungi belonging to Zygomycetes and can be produced by extraction from fungus cell walls. Fungi are thus the promising alternative sources of chitosan. Fungi can be manipulated to give chitosan of more consistent and desired physico-chemical properties compared to chitosan obtained from crustacean sources. In this study, Absidia spp, Aspergillus niger, Rhizopus arrhizus, Cunnighamella elegans, and Mucor rouxii were examined for biomass growth. At first, all five species were grown on synthetic medium at 28 C, 180 rpm in shake-flask incubator. Mucorrouxii which gave the maximum biomass concentration was also grown on molasses. The maximum biomass concentration of Mucor rouxii was found to be higher than that of synthetic medium. The best growth conditions obtained were 4% sucrose, 0.2% yeast extract, 1% peptone and 106 spores in 40 ml. The mycelia harvested at late exponential phase was treated with alkali to remove proteins and chitosan was extracted from cell wall by using acetic acid. The yield of extractable chitosan obtained from cell wall of Mucor rouxii was 2500 mg / l and it is almost 20 % of biomass and approximately 35 % of alkali insoluble fraction.

Konu Başlığı
Chitin -- Biotechnology.
 
Chitosan.
 
Biosynthesis.
 
Fungi Biotechnology.
 
Biotechnological microorganisms -- Isolation.

Yazar Ek Girişi
Harsa, Şebnem

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology and Bioengineering.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Biotechnology and Bioengineering.
 
İzmir Institute of Technology: Biotechnology and Bioengineering--Thesis (Master).

Elektronik Erişim
Access to Electronic Version


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT000249TP248.65.C55 A47 2003Tez Koleksiyonu