Circulating nucleic acid (CNA) separation from serum by electrospun membranes
tarafından
 
Isık, Tuğba, author.

Başlık
Circulating nucleic acid (CNA) separation from serum by electrospun membranes

Yazar
Isık, Tuğba, author.

Yazar Ek Girişi
Isık, Tuğba, author.

Fiziksel Tanımlama
xiii, 63 leaves: color illustraltions.+ 1 computer laser optical disc.

Özet
Early detection of diseases is a key factor that can be made provision and successfully treated. There is a wide array of methods to diagnose cancer like biopsy, endoscopy, magnetic resonance imaging (MRI) and blood tests. In blood, while some nucleic acids are found in intracellular fluids, circulating nucleic acids (CNAs) circulate freely in extracellular fluids. They are located at genomic regions and increased levels of CNAs imply a connection with cancer and tumor initiation. For further analysis, separation and concentration of CNAs have high potential in early cancer detection. A model system was constructed with bovine serum albumin (BSA) and single stranded Deoxyribonucleic acid (ss-DNA) for the investigation of membrane separation efficiency. The membranes made of poly styrene (PS) and poly(methylmethacrylate) (PMMA) were fabricated by electrospinning and they were placed in syringe columns. By using the absorption spectroscopy, the sorption efficiency of membranes was determined. The electrospun membranes are promising for BSA uptake over a wide pH range. Under the same circumstances, thanks to PS fibers, ss-DNA uptake is very with respect to BSA uptake. Our results revealed that PS membranes show a better affinity to BSA molecules by hydrophobic interactions. In the mixture of BSA and ss-DNA, ss- DNA cannot be held on the surface of the membrane and pass through with low sorption efficiency. By altering the membrane amount in syringe column and modifiying the surface of the membranes, the separation could be enhanced. The proposed technology promises fast and effective separation of CNAs from whole blood and body fluids.

Konu Başlığı
Nucleic acids -- Separation.

Yazar Ek Girişi
Demir M. Mustafa

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Materials Science and Engineering.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Materials Science and Engineering.
 
İzmir Institute of Technology: Materials Science and Engineering--Thesis (Master).

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT001360QP620 .I81 2015Tez Koleksiyonu