Development of novel chitosan nanocomposites as a controlled drug release system for helicobacter pylori treatment
Güneş, Suna Seda, author.

Development of novel chitosan nanocomposites as a controlled drug release system for helicobacter pylori treatment

Güneş, Suna Seda, author.

Yazar Ek Girişi
Güneş, Suna Seda, author.

Fiziksel Tanımlama
xiii, 150 leaves:+ 1 computer laser optical disc.

Helicobacter pylori is one of the most common bacterial infection and responsible for gastroduodenal diseases in humans. Due to increasing failure rate of currently used antibiotic therapies, newer drugs and therapeutic approaches are needed. Therefore the use of encapsulated cinnamon bark oil is a promising approach for H. pylori eradication. The main objective of this dissertation was to develop a novel drug delivery system using chitosan and nanoclay containing cinnamon bark oil to be released in a controlled manner in gastrointestinal system for H. pylori eradication as an alternative or complementary to conventional antibiotic treatment. Minimum inhibition concentration (MIC) value of cinnamon bark oil was determined. Cinnamon bark oil loaded chitosan nanocomposite microspheres were produced by spray drying. The prepared microspheres were characterized for particle size & morphology, encapsulation efficiency, surface charge, mucoadhesion, degradation, swelling and drug release. Antimicrobial activity of the microspheres against H. pylori were investigated. In vitro cell viability of fibroblast and gastric epithelial cells were evaluated. In vitro cellular uptake and binding studies of microspheres were investigated by using gastric epithelial cells. MIC value of cinnamon bark oil was found as 8 μg/mL. Nanoclay incorporation decreased the biodegradation of nanocomposite microspheres and improved the release of cinnamon bark oil. Drug release mechanism was anomalous diffusion which refers to combination of diffusion and erosion controlled. The prepared microspheres showed strong mucoadhesive property. Oil released from prepared microspheres inhibited H. pylori growth. Although cinnamon oil showed cytotoxic effect above 31 μg/mL, the oil encapsulated chitosan microspheres did not show any cytotoxic effect on NIH3T3 and MKN45 cells. The prepared microspheres were able to get internalized into MKN45 cells and had great incorporation activity. The results of this study demonstrated that cinnamon bark oil loaded chitosan nanocomposites may serve as biocompatible and effective gastroretentive drug carrier for the treatment of H. pylori infection.

Konu Başlığı
Helicobacter pylori.
Drugs -- Controlled release.
Nanocomposites (Materials).

Yazar Ek Girişi
Tıhmınlıoğlu, Funda

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology and Bioengineering.

Tek Biçim Eser Adı
Thesis (Doctoral)--İzmir Institute of Technology: Biotechnology and Bioengineering.
İzmir Institute of Technology: Biotechnology and Bioengineering (Doctoral).

Elektronik Erişim
Access to Electronic Versiyon.

LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT001511QR82.Z9 G97 2016Tez Koleksiyonu