Investigation of metastatic potential of breast cancer cells in lung-on-a-chip
tarafından
Turgut, Deniz Cemre, author.
Başlık
:
Investigation of metastatic potential of breast cancer cells in lung-on-a-chip
Yazar
:
Turgut, Deniz Cemre, author.
Yazar Ek Girişi
:
Turgut, Deniz Cemre, author.
Fiziksel Tanımlama
:
x, 35 leaves: charts;+ 1 computer laser optical disc.
Özet
:
Breast cancer stands as the most prevalent cancer among women and ranks as the second leading cause of cancer-related fatalities globally. The primary contributor to mortality is metastasis. Triple negative breast cancer exhibits a tendency to metastasize to the lungs, as lungs identified as one of the most common targets of breast cancer metastases. However, to study the precise mechanisms governing this organ-specific metastatic pattern requires a sophisticated 3D lung model, which can truly recapitulate lung physiology and architecture. This study explores the metastatic behaviour of MDAMB-231 breast cancer cells and their interaction with tumour-associated macrophages (TAMs) within a 3D lung model created through a novel biopsy punch technique. The obtained punched lung tissues are integrated into microphysiological systems presenting a hybrid 3D culture model for lung studies. The key findings of this research reveal the effectiveness of biopsy punches in preserving tissue integrity and ensuring a consistent size distribution. Additionally, the study demonstrates the viability of lung tissues for up to 72 hours. Most notably, MDA-MB-231 cells exhibit a significant capacity to invade the punched lung tissues with even greater infiltration observed in the presence of differentiated U-937 cells. These findings provide valuable insights into the metastatic potential of breast cancer cells and highlight the importance of TAMs in facilitating cancer progression within a 3D lung microenvironment. This novel tissue culture technique opens new avenues for studying cancer metastasis, precision medicine and tumour-microenvironment interactions.
Konu Başlığı
:
Metastasis
Cancer cells
Breast -- Cancer
Yazar Ek Girişi
:
Pesen Okvur, Devrim,
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Molekular Biology and Genetics.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology:Molecular Biology and Genetics.
İzmir Institute of Technology: Molekular Biology and Genetics --Thesis (Master)
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası |
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IYTE Library | Tez | T002821 | RC269.5 .T93 2023 |