Investigation of metastatic potential of breast cancer cells in lung-on-a-chip
tarafından
 
Turgut, Deniz Cemre, author.

Başlık
Investigation of metastatic potential of breast cancer cells in lung-on-a-chip

Yazar
Turgut, Deniz Cemre, author.

Yazar Ek Girişi
Turgut, Deniz Cemre, author.

Fiziksel Tanımlama
x, 35 leaves: charts;+ 1 computer laser optical disc.

Özet
Breast cancer stands as the most prevalent cancer among women and ranks as the second leading cause of cancer-related fatalities globally. The primary contributor to mortality is metastasis. Triple negative breast cancer exhibits a tendency to metastasize to the lungs, as lungs identified as one of the most common targets of breast cancer metastases. However, to study the precise mechanisms governing this organ-specific metastatic pattern requires a sophisticated 3D lung model, which can truly recapitulate lung physiology and architecture. This study explores the metastatic behaviour of MDAMB-231 breast cancer cells and their interaction with tumour-associated macrophages (TAMs) within a 3D lung model created through a novel biopsy punch technique. The obtained punched lung tissues are integrated into microphysiological systems presenting a hybrid 3D culture model for lung studies. The key findings of this research reveal the effectiveness of biopsy punches in preserving tissue integrity and ensuring a consistent size distribution. Additionally, the study demonstrates the viability of lung tissues for up to 72 hours. Most notably, MDA-MB-231 cells exhibit a significant capacity to invade the punched lung tissues with even greater infiltration observed in the presence of differentiated U-937 cells. These findings provide valuable insights into the metastatic potential of breast cancer cells and highlight the importance of TAMs in facilitating cancer progression within a 3D lung microenvironment. This novel tissue culture technique opens new avenues for studying cancer metastasis, precision medicine and tumour-microenvironment interactions.

Konu Başlığı
Metastasis
 
Cancer cells
 
Breast -- Cancer

Yazar Ek Girişi
Pesen Okvur, Devrim,

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Molekular Biology and Genetics.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology:Molecular Biology and Genetics.
 
İzmir Institute of Technology: Molekular Biology and Genetics --Thesis (Master)

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer Numarası
IYTE LibraryTezT002821RC269.5 .T93 2023