Molecular and cellular level adaptations of bone marrow mesenchymal progenitor cells to chemical and physical signals
tarafından
Baskan Erbilgiç, Öznur, author.
Başlık
:
Molecular and cellular level adaptations of bone marrow mesenchymal progenitor cells to chemical and physical signals
Yazar
:
Baskan Erbilgiç, Öznur, author.
Yazar Ek Girişi
:
Baskan Erbilgiç, Öznur, author.
Fiziksel Tanımlama
:
xii, 70 leaves: charts;+ 1 computer laser optical disc.
Özet
:
Mechanical forces are the integral determinants in cell and tissue homeostasis and regeneration, and they can affect numerous biological process from proliferation to fate determination. Mechanical forces that possess low magnitude and high frequency characteristics are also known as low intensity vibrations (LIVs). These signals were studied widely on many cell types for regenerative purposes, however most of these studies select components of LIV signals (e.g. magnitude, frequency, duration, etc.) arbitrarily. Here, we addressed the effect of LIV applied frequency, LIV daily exposure time and fate induction on the viability of preadipocyte 3T3-L1 cells. For this, we performed a frequency sweep that was ranging from 30 to 120 Hz with 15 Hz increments applied for 5, 10 or 20 minutes during quiescent growth or adipogenesis for up to 10 days. Results suggest that the applied frequency and fate induction was an important determinant of cell viability, lipid droplet physiology, triglyceride concentration, cell density and adipogenic-specific gene expression while daily exposure time had no effect. These findings contribute to the effort of optimizing a relevant mechanical stimulus that can inhibit adipogenesis. On the other hand, random and aligned PAN/PPy nanofibers were investigated as a scaffold material for osteogenic differentiation of D1 ORL UVA mouse bone marrow mesenchymal stem cells. Cells were able to attach and grow on nanofibers confirmed by cell viability results. Stem cells that were cultured with osteogenic induction were able to mineralize on electrospun nanofibers based on alizarin red and Von Kossa dye staining. For aligned PPy nanofibers, mineralization occurred in the fiber alignment direction. Consequently, PAN/PPy nanofibrous mats in both random and aligned forms would be potential candidates for bone tissue engineering.
Konu Başlığı
:
Bone marrow cells.
Mesenchymal stem cells.
Yazar Ek Girişi
:
Özçivici, Engin,
Atabey, Safiye Neşe,
Tüzel Kişi Ek Girişi
:
İzmir Institute of Technology. Bioengineering.
Tek Biçim Eser Adı
:
Thesis (Master)--İzmir Institute of Technology:Bioengineering.
İzmir Institute of Technology: Bioengineering--Thesis (Master).
Elektronik Erişim
:
Library | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi |
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IYTE Library | Tez | T002287 | QP92 .B315 2020 | Tez Koleksiyonu |