Real-time PCR as a molecular tool for the enumeration of probiotics in commercial products
tarafından
 
Öz, Ödül, author.

Başlık
Real-time PCR as a molecular tool for the enumeration of probiotics in commercial products

Yazar
Öz, Ödül, author.

Yazar Ek Girişi
Öz, Ödül, author.

Fiziksel Tanımlama
xiii, 71 leaves: color illustraltions.+ 1 computer laser optical disc.

Özet
Quantitative Real-Time PCR (qPCR) assays targeting the 16S rDNA was developed as a genus and species specific detection tool for Bifidobacterium and Lactobacillus, and Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus acidophilus LA-5, respectively. Standard curves were established to quantify these probiotic bacteria. The linear regression of standard curves indicated high correlations between the log numbers of pure probiotic culture cells and the Ct values. The assay had a high efficiency and the limit of detection was estimated to be 1.54 ng DNA (corresponding to 104 cells). Results show that qPCR method may be very useful as a rapid, sensitive and specific tool for detecting and quantifying B. animalis subsp. lactis BB-12 and L. acidophilus LA-5 in probiotic supplements. FTIR spectroscopy was used for the first time to determine the ratios of different microorganisms in commercial probiotic supplements. FTIR analysis was also performed for the pure probiotic cultures of B. animalis subsp. lactis BB-12 and L. acidophilus LA-5. Results obtained in this study showed that FTIR spectroscopy is potentially a rapid method for determining probiotic cell components and their ratios in the supplements and verification their detection and identification.

Konu Başlığı
Probiotics.
 
Polymerase chain reaction.

Yazar Ek Girişi
Baysel, Ayşe Handan

Tüzel Kişi Ek Girişi
İzmir Institute of Technology. Biotechnology and Bioengineering.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology: Biotechnology and Bioengineering.
 
İzmir Institute of Technology: Biotechnology and Bioengineering--Thesis (Master).

Elektronik Erişim
Access to Electronic Versiyon.


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT001474QR171.I6 O99 2016Tez Koleksiyonu