Isolation, characterization, determination of probiotic properties of lactic acid bacteria from human milk
tarafından
 
Yavuzdurmaz, Hatice.

Başlık
Isolation, characterization, determination of probiotic properties of lactic acid bacteria from human milk

Yazar
Yavuzdurmaz, Hatice.

Yazar Ek Girişi
Yavuzdurmaz, Hatice.

Yayın Bilgileri
[s.l.]: [s.n.], 2007.

Fiziksel Tanımlama
xi, 69 leaves.: ill. + 1 computer laser optical disc.

Özet
Probiotics mean live microorganisms that have beneficial effects on their host.s health. Although probiotic strains can be isolated from many sources; for human applications the main criteria is being human origin.Breast milk is an important nutrient source for neonates. Lots of studies showed that this fluid has beneficial effects on the health of neonates. One reason of being beneficial is explaining by the microflora of human breast milk including beneficial lactic acid bacteria.In this study, isolates were identified by biochemical and molecular characterization and also probiotic properties of lactic acid bacteria, isolated from human milk were investigated.Three of the isolates were observed as potential probiotic. Two of them are bacilli and the other is cocci. These isolates showed resistance to stomach pH (pH 3,0), tolerance against 0,3% bile concentration and antimicrobial activity against Salmonella thyphimurium CCM 5445, Escherichia coli O157:H7 NCTC 129000 and Escherichia coli NRRL B-3008. After investigation the probiotic properties of these isolates, they were identified by biochemical characterization techniques and molecular identification by using amplification and restriction fragment length polymorphism (RFLP) of 16S ribosomal DNA (rDNA) and 16S sequencing. Two lactobacilli were identified as Lactobacillus oris and Lactobacillus fermentum. In the light of this study, it is observed that, human milk is a source of potential probiotic strains.

Konu Başlığı
Lactic acid bacteria.
 
Breast milk.
 
Probiotics.

Yazar Ek Girişi
Harsa, Şebnem.

Tüzel Kişi Ek Girişi
Izmir Institute of Technology. Food Engineering.

Tek Biçim Eser Adı
Thesis (Master)--İzmir Institute of Technology:Food Engineering.
 
İzmir Institute of Technology:Food Engineering--Thesis (Master).

Elektronik Erişim
Access to Electronic Version.


LibraryMateryal TürüDemirbaş NumarasıYer NumarasıDurumu/İade Tarihi
IYTE LibraryTezT000712QR121 .Y35 2007Tez Koleksiyonu