Natural and synthetic silica incorporated chitosan composite scaffolds for bone tissue engineering applications için kapak resmi
Natural and synthetic silica incorporated chitosan composite scaffolds for bone tissue engineering applications
Tamburacı, Sedef, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xv, 158 leaves: color illustraltions.+ 1 computer laser optical disc
Recently bone tissue engineering studies have focused on the development of 3D scaffolds that can organize the tissue regeneration in natural way with appropriate porosity and reinforced the structure. Natural polymer-based composites have been focused with more attention than synthetic polymer composites for bone tissue engineering applications because of their biocompatibility and biodegradability. In this work, the goal was to combine the useful biomaterial properties of both chitosan and silica to design biocomposite organic/inorganic biomaterials for bone tissue engineering applications. The composite scaffolds were fabricated by freeze drying method bu using two different silicas; natural silica; Diatomite and synthetic silica, octa (tetramethylammonium) polyhedral oligomeric silsesquioxanes (OctaTMA-POSS). The effects of silica type and loading on the mechanical, morphological, chemical, surface properties, wettability and biocompatibility of composite scaffolds were investigated and characterized by using SEM, AFM, contact angle analysis, swelling study, protein adsorption assay, biodegradation and biomineralization tests. WST-1 cytotoxicity, cell proliferation with rezasurin and alkaline phosphatase activity assays were performed to determine biological activity of the composite scaffolds. In vitro biomineralization on scaffolds was determined by Von Kossa and Alizarin red staining. POSS and diatomite incorporation increased the surface roughness. Chitosansilica composites exhibited 82-90% porosity. Wet chitosan-silica composite scaffolds exhibited higher compression moduli compared to pure chitosan scaffold in 67.3- 81.4kPa and 78.1 to 107.6kPa range respectively. Average pore size range of chitosandiatomite and chitosan-POSS composite scaffolds was obtained as 15-180μm and 220- 300μm, respectively. Results indicated that chitosan-silica composites did not show any cytotoxic effect on 3T3, MG-63 and Saos-2 cell lines. Chitosan-silica composites were found to be favorable for osteoblast proliferation. Diatomite and POSS incorporation showed promising effects with enhancing ALP activity on hFob cells. Therefore, these composite scaffolds could be used for bone tissue engineering applications.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Doctoral)--İzmir Institute of Technology: Biotechnology and Bioengineering.

İzmir Institute of Technology: Biotechnology and Bioengineering (Doctoral).
Elektronik Erişim:
Access to Electronic Versiyon.


Materyal Türü
Demirbaş Numarası
Yer Numarası
Durumu/İade Tarihi
Tez T001508 R857.M3 T15 2016

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