Immobilization of thermophilic esterase on magnetic cornstarch nanoparticles for biological applications
Başlık:
Immobilization of thermophilic esterase on magnetic cornstarch nanoparticles for biological applications
Yazar:
Öz, Yasin, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
x, 56 leaves:+ 1 computer laser optical disc.
Özet:
In last three decades, even the role of enzymes for biological and industrial applications has become more worthy, enzymes also have some defects. The enzyme immobilization allows to overcome these defects by improving abilities of reusing of catalysts by multiple times, easier reactor operation and product separation. Due to its potential use in biological and industrial applications, isolated thermophilic esterase from Geobacillus sp. was immobilized on magnetic cornstarch nanoparticles. In order to determine activity performance of immobilized enzyme, the effects of temperature, pH and some chemicals on enzyme activity were investigated. The results have shown that after immobilization, the relative activity of immobilized esterase has increased to 80% at 80 0C in comparison to free esterase. Therewithal, the reusability of immobilized esterase has increased fourfold in comparison to free esterase. The magnetic character of the support media has brought ease to separate biocatalysts from reaction media.
Yazar Ek Girişi:
Tüzel Kişi Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Chemistry.
İzmir Institute of Technology: Chemistry--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.