Investigation of the effects of SIV AGM Tat protein on SLPI gene expression in primate cells için kapak resmi
Investigation of the effects of SIV AGM Tat protein on SLPI gene expression in primate cells
Demirci, Dilara, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xi, 60 leaves: illustrarions, charts;+ 1 computer laser optical disc.
Old World monkeys one of which is African Green monkey (AGM) have resistance against to HIV-1. They can be infected by species-specific simian immunodeficiency viruses (SIV), however they do not develop AIDS-like syndrome. There are several restriction factors already known that prevent HIV-1 replication protecting these organisms from infection. However, although Old World monkey cells can be successfully infected with HIV-1 by using high viral loads, the virus eventually become undetectable, indicating the presence of yet unidentified anti HIV-1 factor(s) that confer resistance to these cells. Old world monkeys may have evolved resistance mechanisms by switching specific genes on in response to the synthesis of viral proteins. Since HIV-1 Tat protein is the first viral protein synthesized after viral entry and its presence is required for the synthesis of other HIV-1 proteins, it may also play an important role on the activation of such resistance mechanism. In the previous studies conducted in our laboratory, Secretory Leukocyte Protease Inhibitor (SLPI) which is a 11,7 kDa protein with antiprotease, antimicrobial and antiviral activities was identified as a candidate restriction factor. It was observed to be overexpressed in AGM cell lines in the presence of HIV tat. Although, HIV Tat and SIV Tat have similar functions in the infected cells, they are highly dissimilar in their aminoacid composition (≈40% similarity). Therefore the effects of HIV-1 Tat and SIV Tat on SLPI gene expression might be different. The aim of this study was to investigate the effects of SIV agm Tat on SLPI expression in AGMcells. For this purpose, CV-1 cell lines stably expressing SIV tat gene were established and the expression of SLPI was determined by Q-PCR and Western blotting. A slight decrease in SLPI expression in stable monkey cells that express SIV Tat was observed while HIV Tat expressing stable cells showed SLPI overexpression. iv
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.

İzmir Institute of Technology: Molecular Biology and Genetics.--Thesis (Master).
Elektronik Erişim:
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Durumu/İade Tarihi
Tez T001602 QH450 .D378 2017

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