Molecular genetic analyis in faba bean (Vicia faba L.) için kapak resmi
Molecular genetic analyis in faba bean (Vicia faba L.)
Abuzayed, Mazen Ali, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xiv, 103 leaves: illustrarions, charts;+ 1 computer laser optical disc.
Faba bean (Vicia faba L.) is an important food legume crop with a huge genome. In this study, we used Next Generation Sequencing (NGS) technology for development of genomic simple sequence repeat (SSR) markers. A total of 14,027,500 sequence reads were obtained comprising 4,208 Mb. From these reads, 56,063 contigs were assembled (16,367 Mb) and 2138 SSRs were identified. Mono and dinucleotides were the most abundant, accounting for 57.5% and 20.9% of all SSR repeats, respectively. A total of 430 primer pairs were designed from contigs larger than 350 nucleotides and 50 primers pairs were tested for validation of SSR locus amplification. Nearly all (96%) of the markers were found to produce clear amplicons and to be reproducible. Thirty-nine SSR markers were then applied to 46 faba bean accessions from worldwide origins, resulting in 161 alleles with 87.5% polymorphism, and an average of 4.1 alleles per marker. Gene diversity (GD) of the markers ranged from 0.00 to 0.48 with an average of 0.27. Testing of the markers showed that they were useful in determining genetic relationships and population structure in faba bean accessions. In addition, 26 morphological and seven biochemical (phenolics content, flavonoids, protein, L-DOPA, tannins, vicine and convicine) characters of 61 landraces and 53 faba bean cultivars were analyzed. There was high diversity for the studied characters among the accessions. Association mapping for these morphological and biochemical characters with 59 SSR markers (442 fragments) was conducted using a general linear model based on the Q matrix. As a result, 48 significant loci were detected for 22 morphological characters, and 26 loci were detected for six biochemical traits. The range of LD (r2) was from 0.09 to 0.18, and from 0.06 to 0.13 for morphological and biochemical associations, respectively. This study can help breeding programs in selection and improvement of faba bean production.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Doctoral)--İzmir Institute of Technology: Molecular Biology and Genetics.

İzmir Institute of Technology: Molecular Biology and Genetics--Thesis (Doctoral).
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Materyal Türü
Demirbaş Numarası
Yer Numarası
Durumu/İade Tarihi
Tez T001993 QK981.4 .A167 2019
Tez ROM3153 QK981.4 .A167 2019 EK.1

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