Chemical characterization of caldanaerobacter subterraneus subsp. tengcongensis heme-nitric oxide/oxygen binding protein
Başlık:
Chemical characterization of caldanaerobacter subterraneus subsp. tengcongensis heme-nitric oxide/oxygen binding protein
Yazar:
Erdal, Merve, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xi, 61 leaves: charts;+ 1 computer laser optical disc.
Özet:
Hemoproteins, which contain the heme prosthetic group , take part in different biological processes in many stages of life. Their ability to catalyze important biosynthesis reactions makes them good candidates for understanding and elucidating complex mechanisms for biocatalysis. In this study, the catalytic properties of thermophilic Thermoanaerobacter tencogensis nitric oxide/oxygen binding protein, a heme protein reshaped by rational design, were investigated and chemical characterization was carried out. The peroxidase activity of the enzyme was investigated by the oxidation reactions of guaiacol, amplex red and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS). Kinetic parameters of the reactions were determined. These obtained results demonstrated that, in the presence of H2O2, wild type and Y140H TtH-NOX proteins are able to catalyze oxidation reactions of guaiacol, Amplex red and ABTS. Comparison of the kinetic parameters showed that Y140H mutant catalyzed the guaiacol and ABTS oxidation 3-fold and 15 -fold faster than wild type enzyme, respectively. The stability of TtH-NOX proteins were investigated in the presence of organic solvents. Results were demonstrated that WT TtH-NOX was more stable than Y140H mutant in the presence of organic solvents In addition to these, for the first time, thermophilic TtH-NOX proteins were immobilized with a novel enzyme immobilization method and organic-inorganic hybrid nanostrucrures were obtained. Copper ion incorporated TtH-NOX-based hybrid nanoflowers were synthesized at different pH values. SEM and EDX analysis of TtH-NOX-based hybrid nanoflowers proved that free TtH-NOXs were immobilized successfully.
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology:Bioengineering.
İzmir Institute of Technology: Bioengineering --Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.