Induction of hairy root culture in eggplant with rhizobium rhizogenes için kapak resmi
Induction of hairy root culture in eggplant with rhizobium rhizogenes
Öztürk, Tuba, author.
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Fiziksel Tanımlama:
ix, 54 leaves: charts;+ 1 computer laser optical disc.
Eggplant is an economically and agronomically important crop. This crop has nutritional value and many health effects come from its secondary metabolites. Due to constraints in conventional methods, Rhizobium rhizogenes-mediated plant transformation provides an alternative way to produce secondary metabolites. The bacterium causes hairy root disease via the integration of its T-DNA into the plant genome. In this study, we used two agropine strains (ATCC 15834 and ATCC 43057) of R. rhizogenes to compare hairy root induction in hypocotyl and cotyledon explants of eggplant. Transformation frequency was found to be the highest in hypocotyl explants (49.4%) infected with ATCC 15834. Thirty nine hairy root clones were selected based on their hairy root phenotype. The transgenic status of 15 of these clones was tested by PCR using rol gene primers. Hairy roots infected with ATCC 15834 were grown for 8 weeks in MS liquid medium. At the end of this growth period, the growth ratio of the hairy roots was measured. The root biomass was 6.52 g/DW for hypocotyl and 9.83 g/DW for cotyledon-derived clones. Biochemical analyses of clones were performed. Total phenolic contents were evaluated for hypocotyl and cotyledon-derived clones yielding 8.56 mg/g DW and 8.81 mg/g DW, respectively. The average total flavonoid content was 2.12 mg/g DW in hypocotyl-derived clones, and 1.90 mg/g DW in cotyledon-derived clones. On average, these amounts of both phenolic and flavonoid contents were significantly higher than those obtained for untransformed eggplant roots. Overall the results indicated that eggplant hairy roots are a useful source of secondary metabolites. In the future, the production of metabolites of interest such as steroidal glycoalkaloids or phenolics can be improved using elicitors in eggplant.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology:Molecular Biology and Genetics.

İzmir Institute of Technology: Molekular Biology and Genetics --Thesis (Master).
Elektronik Erişim:
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Tez T002349 QK981 .O99 2021

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