Preparation and characterization of drug loaded cationic albumin nanoparticles için kapak resmi
Preparation and characterization of drug loaded cationic albumin nanoparticles
Sözer, Sümeyra Çiğdem, author.
Fiziksel Tanımlama:
x, 52 leaves: charts;+ 1 computer laser optical
Serum albumin protein behaves as a carrier and transporter for both hydrophilic and hydrophobic drugs. Therefore, albumin could be used in the drug carrier systems. Since albumin nanoparticles have a negative charge under physiological conditions, their anionic drug loading and delivering capacities are restricted. This study aims to obtain higher anionic drug loading capacity by producing cationic bovine serum albumin nanoparticles (cBSA NPs). Firstly, the carboxyl groups of amino acids present on the surface of albumin were conjugated with ethylenediamine to change the charge of albumin from negative to positive. Then, cBSA NPs were obtained using the desolvation process. Anionic salicylic acid (SA) was used for drug loading studies of the obtained cBSA NPs. SA loading and releasing experiments were studied with UV-Vis and electron paramagnetic resonance (EPR) spectroscopy. In the UV-Vis, the drug loading capacity of cBSA NPs was found to increase ~2 fold, and drug release was slower compared to BSA NPs. For EPR studies, SA was labeled with stable radicals. Spin labels allow the simultaneous monitoring of bound and free drugs in the same sample. The drug was loaded into nanoparticles using two methods. Based on EPR results, it was found that drug was loaded to cBSA NPs with 50% and 93%, and to BSA NPs with 4% and 15% ratios, by desolvation and incubation, respectively. Thus, UV-vis and EPR measurements showed that cBSA NPs have higher SA loading potential and slower release ability compared to anionic albumin nanoparticles.
Konu Başlığı:

Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology:Materials Science and Engineering.

İzmir Institute of Technology: Materials Science and Engineering--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.


Materyal Türü
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Durumu/İade Tarihi
Tez T002362 TA418.9.N35 S73 2021

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