Genetic modification of chlorogenic acid content in eggplant (Solanum melongena l.) by Agrobacterium-mediated transformation technique için kapak resmi
Genetic modification of chlorogenic acid content in eggplant (Solanum melongena l.) by Agrobacterium-mediated transformation technique
Tanyolu, Tanya Nil, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
ix, 57 leaves: charts;+ 1 computer laser optical disc.
Eggplant is a nutritious vegetable that is low in lipids and high in fiber, vitamins, and minerals. Eggplant is a good source of phenolic compounds, particularly chlorogenic acid (CGA), which is associated with several health benefits, such as reducing the risk of diabetes and cardiovascular diseases. Among CGA-rich fruits, eggplant has a geographically wide distribution while coffee is limited to tropical regions. For that reason, eggplant can be used as alternative to coffee for CGA production. The goal of this research was to develop transgenic eggplant that has increased CGA content using the Agrobacterium-mediated transformation technique. The over-expression of the Myb1 gene, which is related to CGA synthesis and responsible for the production of a transcription factor that activates the phenylpropanoid pathway in eggplant, was used. The Myb1 gene was isolated, amplified, and inserted into the PRI910 vector system before being transformed into Agrobacterium tumefaciens. After plant transformation, seven regenerated plants were verified as transgenic by PCR analysis. Young leaves from transgenic eggplants were used for mRNA expression analysis and metabolite analyses. A two-fold increase in Myb1 gene expression levels for three of the transformed plants was observed and five plants showed a significant increase in 3-CGA content. Expression of Myb1 correlated with CGA content measured by HPLC analysis. Specifically, the two-fold increases in Myb1 expression of three plants corresponded with two-fold increases in their leaf 3- CGA content. In the future, these genetically modified plants could serve a crucial role in better understanding the pathway of CGA production in eggplants, and may also prove useful in generating valuable CGAs for both economic and medicinal purposes. Subsequent research can be directed towards determining the CGA content via measurement of 5-CGA and examining CGA content in different parts of the fruit such as flesh and peel. Study of the next generation (T1 plants) will also be critical for confirming the stability of the transformation.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology:Molecular Biology and Genetics.

İzmir Institute of Technology: Molekular Biology and Genetics --Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.


Materyal Türü
Demirbaş Numarası
Yer Numarası
Durumu/İade Tarihi
Tez T002765 XX(2342594.1)

On Order