Blood-brain-barrier-on-chip model comprising mouse brain tissue

The brain blood barrier (BBB) has great importance in the pathologies of brain diseases and in treatment approach studies such as drug delivery. Modelling the blood brain barrier with microfluidics with high-throughput and realistic designs is an effective option for reducing animal use in research, disseminating precision medicine, and advancing scientific research. In this study, it was aimed to develop a BBB model by containing adult mouse brain tissue. For this purpose, a tool and method has been developed that will enable to obtain brain tissue in approximately equal size and small enough to be perusable for nutrition. The microfluidic model suitable for the BBB model has been developed and its properties have been optimized according to the progress of the study. The parameters for tissue viability were optimized and the tissue lived in the microfluidic. Microvascular network formations of tissue-containing and non-tissue 3D environments were investigated with the inclusion of different components. The functional state of the BBB model was tested by permeability assay after construction the optimized vascular network and endothelial cell layer.