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Development and characterization of surface-modified emulsion templated scaffolds for tissue engineering applications için kapak resmi
Development and characterization of surface-modified emulsion templated scaffolds for tissue engineering applications
Başlık:
Development and characterization of surface-modified emulsion templated scaffolds for tissue engineering applications
Yazar:
Kocagöz, Mehmet, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xvi, 112 leaves: illustrarions, charts; 29 cm + 1 computer laser optical disc.
Özet:
Emulsion templating is an advantageous scaffold fabrication method that provides high and open porosity. In this method, water-in-oil emulsions are obtained by mixing hydrophobic polymers and water. Polymerized emulsions with an internal phase greater than 74% are named high internal phase emulsions (PolyHIPEs). Polycaprolactone is a synthetic, biodegradable and biocompatible polymer widely used in tissue engineering, but its hydrophobicity limits cell-material interactions. Accordingly, this study aims to develop and characterize the NaOH-treated emulsion templated polycaprolactone tetramethacrylate (4PCLMA)-based scaffolds to improve its biological performance. Firstly, 4PCLMA pre-polymer was synthesized by ring-opening polymerization and functionalized with methacrylate groups. 4PCLMA was successfully synthesized with ~97% degree of methacrylation. 4PCLMA PolyHIPEs were fabricated by emulsion templating and post-treated with sodium hydroxide (NaOH) at three different concentrations and incubation times. The effects of NaOH treatment on mass loss, water absorption capacity, mechanical characteristics, surface area, hydrophilicity and biological performance of the scaffolds were investigated. The morphologies of the scaffolds were investigated using SEM. NaOH treatment reduced the weight and mechanical strength of the scaffolds, but it also increased the water absorption capacity, hydrophilicity, surface area and protein adsorption of the scaffolds. Chemical changes in PolyHIPEs after NaOH treatment were confirmed via spectroscopy. In vitro results showed that NaOH treatment did not cause cytotoxicity on L929 cells and positively affected the cell attachment and proliferation behaviour of Saos-2 cells. As a result of this study, NaOH treatment was investigated as an alternative surface modification technique to improve the hydrophilicity and biological performance of emulsion-templated scaffolds.
Tek Biçim Eser Adı:
Thesis (Master)-- İzmir Institute of Technology: Bioengineering.

İzmir Institute of Technology: Bioengineering. (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
Ayırtma: Copies: