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Development of 3D tumor models for investigating drug efficacy of sapogenol derivatives için kapak resmi
Development of 3D tumor models for investigating drug efficacy of sapogenol derivatives
Başlık:
Development of 3D tumor models for investigating drug efficacy of sapogenol derivatives
Yazar:
Pişirici, Necmettin Arda, author.
Fiziksel Tanımlama:
xvii, 147 leaves: charts;+ 1 computer laser optical disc.
Özet:
In studies on the treatment of cancer, which is a common disease of the latest period, 3D cell culture models, which show high similarity to tissue physiology, attract attention in order to investigate the efficacy of potential drugs. These models are preferred in drug development studies by overcoming many problems in 2D cell culture studies. In the completed research, drug activity screenings of sapogenol-derived molecules and anticancer drug Ptx were performed on 3D tumor spheroid models produced using new generation magnetic levitation (MagLev) technology. The magnetic levitation is a scaffold free methodology which allows the cells magnetized by the effect of paramagnetic agents in the magnetic field created by two magnets to balance at a unique height (levitation height) depending on density, without requiring an external force. With this method, intercellular interactions with the same level of levitation heights can be increased and 3D spheroid structures can be obtained quickly without the need any scaffold. In the completed thesis; As a control group for MagLev spheroids, hanging drop methodology was preferred with the same cell lines. The activity of sapogenol-derived molecules and the Ptx were investigated. The expected toxic effect on spheroids was observed and comparative results were shared. MagLev based new drug activity screening model which overcomes the disadvantages of 2D cell culture studies, can imitate real tumor physiology much more closely, can provide easy, realistic and fast results in drug screenings and investigating the effects of different molecules before clinical studies has been devoloped
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology:Bioengineering.

İzmir Institute of Technology: Bioengineering --Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
Ayırtma: Copies: