In vitro evaluation of poly (2-((2-aminoethyl) Amino) ethyl methacrylate) as a potential siRNA delivery agent
Başlık:
In vitro evaluation of poly (2-((2-aminoethyl) Amino) ethyl methacrylate) as a potential siRNA delivery agent
Yazar:
Zelçak, Aykut, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
x, 68 leaves:+ 1 computer laser optical disc.
Özet:
The aim of this thesis is to investigate poly(2-((2-aminoethyl)amino)ethyl methacrylate) (P(AEAEMA)) as a potential siRNA carrier. For this purpose, an amine containing monomer 2-((tert-butoxycarbonyl) (2-((tert-butoxy carbonyl) amino) ethyl) amino) ethyl methacrylate (BocAEAEMA) was synthesized. Reversible addition-fragmentation chain transfer (RAFT) polymerization was performed to prepare homo- and block co-polymers of BocAEAEMA. The synthesized polymers -P(AEAEMA)19, P(AEAEMA)41 and P(PEGMA)12-b-P(AEAEMA)32- were characterized via NMR and GPC. The cytotoxicity of the polymers was investigated in vitro using ovarian cancer cell line (Skov-3-luc) via MTT assay. The polymers did not show any toxic effect on cells in 24 h. The ability of the BocAEAEMA polymers to form polyplexes with siRNA was investigated via gel electrophoresis. P(AEAEMA)19, P(AEAEMA)41 and P(PEGMA)12-b-P(AEAEMA)32 could efficiently form complexes with siRNA at an N/P ratio of 5, 2, and 2 respectively. Gel electrophoresis analysis revealed that P(AEAEMA)41 and P(PEGMA)12-b-P(AEAEMA)32 could protect siRNA against serum components at least for 6 h. Block copolymer, when complexed with siRNA at an N/P ratio of 10, could protect siRNA longer (24 h) when compared with the homopolymer. The size and surface charge of the polyplexes were investigated by DLS. The diameter of the P(AEAEMA)41-siRNA complexes was found to be lower than 110 nm at all N/P ratios tested. In contrast, P(PEGMA)12-b-P(AEAEMA)32-siRNA complexes (except the complex prepared at the N/P ratio of 2), displayed aggregation tendency. All polyplexes displayed positive zeta potential. The zeta potential of the homopolymer was found to be higher than the copolymer at the N/P ratio of 2. Finally, in order to determine siRNA transfection ability of the polymers, luciferase assay was optimized using a commercial transfection reagent lipofectamine RNAimax. The optimized assay conditions will be used in future studies to determine the transfection efficiency of the polymers.
Yazar Ek Girişi:
Tüzel Kişi Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Biotechnology and Bioengineering.
İzmir Institute of Technology: Biotechnology and Bioengineering--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.