Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins için kapak resmi
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Başlık:
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Yazar:
Danis, Clément
Yazar Ek Girişi:
Yayın Bilgileri:
Cambridge, MA MyJoVE Corp 2016
Fiziksel Tanımlama:
online resource (767 seconds)
Seri:
Biochemistry
Genel Not:
Title from resource description page
Özet:
We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.
Okuma Düzeyi:
For undergraduate, graduate, and professional students
Konu Başlığı:
Elektronik Erişim:
https://www.jove.com/t/55001
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