
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Başlık:
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Yazar:
Danis, Clément
Yazar Ek Girişi:
Yayın Bilgileri:
Cambridge, MA MyJoVE Corp 2016
Fiziksel Tanımlama:
online resource (767 seconds)
Seri:
Biochemistry
Genel Not:
Title from resource description page
Özet:
We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.
Okuma Düzeyi:
For undergraduate, graduate, and professional students
Konu Başlığı:
Elektronik Erişim:
https://www.jove.com/t/55001